常规说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
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存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
纯化说明 This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The Abpromise guarantee
Abpromise™承诺保证使用ab6645于以下的经测试应用
\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution + Beta Galactosidase at 0.05 µg with Blocking buffer
Secondary
HRP streptavidin conjugate at 1/40000 dilution
发表研究结果有使用 ab6645？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab6645 被引用在 4 文献中.
Matsumoto K et al. In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay). Sci Rep 7:12136 (2017).PubMed: 28939819 Herrera JJ et al. Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury. J Neurotrauma 27:2067-76 (2010). IHC-FrPubMed: 20799882 Siembab VC et al. Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells. J Comp Neurol 518:4675-701 (2010).PubMed: 20963823 Glaser T et al. Generation of purified oligodendrocyte progenitors from embryonic stem cells. FASEB J 19:112-4 (2005).PubMed: 15486057I would recommend ab6645. We do not know if this antibody has been used in formalin treated tissue, however it has been used with IHC and I would expect it to work with formalin treated tissue as it is a polyclonal and thus recognises multiple epitopes. Read More
This antibody has not yet been tested in Flow Cytometry, therefore we can\'t guarantee that it will work. For a protocol regarding the staining of red blood cells, please see the general FACS protocol on the Abcam website
Read MoreThis antibody is biotinylated so there will be no need for a secondary antibody. You only require an ABC kit and chromogen. I recommend ab8621 for the ABC kit (Super ABC / Horseradish Peroxidase Basic Kit) and ab8184 (HRP Chromogen kit (DAB)) for the chromogen.I will personally e-mail you my antigen retrieval protocol. Try the pressure cooker method for starters.
Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
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