Alkaline Phosphatase抗体(ab354)| Abcam中文官网

来自 : 发布时间：2024-05-07

Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli].
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存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
纯化说明 This product was prepared from monospecific antiserum by a delipidation and defibrination.
Alkaline phosphatase, germ cell antibody Alkaline phosphatase, testicular and thymus antibody Akp2 antibody
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发表研究结果有使用 ab354？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab354 被引用在 5 文献中.

Hemnes AR et al. BMPR2 dysfunction impairs insulin signaling and glucose homeostasis in cardiomyocytes. Am J Physiol Lung Cell Mol Physiol 318:L429-L441 (2020).PubMed: 31850803 Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018).PubMed: 30043216 Bross P et al. The HSP60-(P.val98ile) mutation associated with hereditary spastic paraplegia SPG13 compromises chaperonin function both in vitro and in vivo. J Biol Chem : (2008). Escherichia coliPubMed: 18400758 Imperi F et al. Membrane-association determinants of the omega-amino acid monooxygenase PvdA, a pyoverdine biosynthetic enzyme from Pseudomonas aeruginosa. Microbiology 154:2804-13 (2008).PubMed: 18757814 Downer R et al. The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein. J Biol Chem 277:243-50 (2002).PubMed: 11684686
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) Concentration: 5% Temperature: RT C
Blocking step Milk as blocking agent for 30 minute(s) Concentration: 5% Temperature: RT C

Thank you for your enquiry. This product is sold as tissue culture supernatant and not affinity purified. As a tissue culture supernatant this product will contain additional proteins and the IgG concentration has not been determined. Typically IgG concentrations in tissue culture supernatant will range between 0.5 and 1 mg/ml. If you have any further questions then please get back in touch with us.
This antibody should detect SEAP from E.coli. It has not been tested for cross-reactivity in other bacteria and does not cross-react with AP from mammalian species. Read More

A working dilution from our test using lot 4679(assayed againest 1.0 ug of Alkaline Phosphatase in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anit- Rabbit IgG, was 1:8,000 to 1:40,000 (volume:volume).

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No this antibody is not suitable as it does not cross react with the mammalian form of Alkaline Phosphatase. The immunogen is Alkaline Phosphatase from E.coli. We suggest you review our other alkaline phosphatase antibodies, many of which have been tested in IHC. Read More

No, this antibody is not a marker of plasma membrane.

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We have no reports of it being used in this application, but do not see a reason why it should not recognise SEAP.

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We believe it is although it might be more highly expressed in stationary phase and be strain dependent. Abcam

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