FITC荧光beta Actin抗体[AC-15]| Abcam中文官网

来自 : 发布时间：2024-05-07

Synthetic peptide:DDDIAALVIDNGSGLconjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). Run BLAST withRun BLAST with 常规说明 Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cellsThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As Primary antibody说明 Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells The Abpromise guarantee Abpromise™承诺保证使用ab6277于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. 疾病相关 Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. 细胞定位 Cytoplasm cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 30 min blocking with TBS-T / 5% BSA1 hr incubation with ab6277 (TBS-T / 5% BSA)1 minute nuclear counterstaining with DAPIMagnification x20 Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277). ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 30 min blocking with TBS-T / 5% BSA1 hr incubation with ab6277 (TBS-T / 5% BSA)1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution. 发表研究结果有使用 ab6277？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab6277 被引用在 17 文献中. Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019).PubMed: 30595434 Réu P et al. A 61% lighter cell culture dish to reduce plastic waste. PLoS One 14:e0216251 (2019).PubMed: 31039189 Cagnetta R et al. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron 99:29-46.e4 (2018).PubMed: 30008298 Olthoff JT et al. Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun 9:5104 (2018).PubMed: 30504831 Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015).PubMed: 26134519 View all Publications for this product Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 1% Temperature: 24 CPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com