Abcam/重组人PLK1蛋白（ab51426）/1/ab51426
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联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Product name
Recombinant human PLK1 proteinSee all PLK1 proteins and peptidesBiological activity
Specific activity 17nmol/min/mg.Expression system
Baculovirus infected Sf9 cellsProtein length
Full length proteinAnimal free
NoNature
RecombinantSpecies
HumanTags
His tag N-Terminus
Associated products
Related Products
- Anti-PLK1 antibody [35-206] (ab17056)
- Anti-PLK1 antibody [36-298] (ab17057)
Substrate reagent
- Native Cow Casein protein (ab91090)
Specifications
Our Abpromise guarantee covers the use ofab51426in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Western blot
Functional Studies
SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Form
LiquidAdditional notes
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Alternative names
- Cell cycle regulated protein kinase
- PLK
- PLK 1
see allFunction
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.Tissue specificity
Placenta and colon.Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.Contains 2 POLO box domains.Contains 1 protein kinase domain.Developmental stage
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.Post-translationalmodifications
Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.- Information by UniProt
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
