Abcam/重组小鼠TNF-α蛋白（活性）（ab259411）/1/活性）（ab259411
商品编号：
Active)(ab259411
品牌：
Abcam
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Product name
Recombinant mouse TNF alpha protein (Active)See all TNF alpha proteins and peptidesBiological activity
Fully active compared to a standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 1.12ng/mL corresponding to a Specific Activity of 8.93x 105 IU/mg.
Purity
>= 95% SDS-PAGE.Purity by HPLC >=95%.Endotoxin level
<>Expression system
HEK 293 cellsAccession
P06804Protein length
Full length proteinAnimal free
YesCarrier free
YesNature
RecombinantSpecies
MouseSequence
GPQRDEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLE WLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLL THTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQL EKGDQLSAEVNLPKYLDFAESGQVYFGVIALPredicted molecular weight
20 kDaMolecular weight information
M + 1.5 Da (Calc mass 19797.5 Da).GPQRDEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIALAmino acids
57 to 235Additional sequence information
N-terminal Glycine
Associated products
Specifications
Our Abpromise guarantee covers the use ofab259411in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Cell Culture
SDS-PAGE
Functional Studies
HPLC
Mass Spectrometry
Form
LyophilizedAdditional notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.
General Info
Alternative names
- APC1
- APC1 protein
- Cachectin
see allFunction
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).Sequence similarities
Belongs to the tumor necrosis factor family.Post-translationalmodifications
The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.Cellular localization
Secreted and Cell membrane.- Information by UniProt
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
