Description
Product name
Recombinant human IGF1 protein (Active)See all IGF1 proteins and peptidesBiological activity
Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.
ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
Purity
>= 95% Immunogen affinity purified.Purity by HPLC >=95%.Endotoxin level
<>Expression system
HEK 293 cellsAccession
P05019Protein length
Full length proteinAnimal free
YesCarrier free
YesNature
RecombinantSpecies
HumanSequence
GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAPredicted molecular weight
8 kDaMolecular weight information
M - 2.84 Da (Calculated mass 7711.84 Da)GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAAmino acids
49 to 118Additional sequence information
N-terminal glycineFull-length mature chain lacking the signal peptide and both pro-peptides.
Associated products
Specifications
Our Abpromise guarantee covers the use ofab270062in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Cell Culture
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
Form
LyophilizedAdditional notes
This protein is filter sterilized prior to aliquoting and lyophilization. All aliquoting and lyophilization steps are performed in a sterile environment
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.
General Info
Alternative names
- IBP1
- IGF I
- IGF IA
see allFunction
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.Sequence similarities
Belongs to the insulin family.Cellular localization
Secreted.- Information by UniProt
