Description
Product name
Recombinant human Hsp90 beta protein (Active)See all Hsp90 beta proteins and peptidesBiological activity
1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.
2. The protein concentrations (where applicable) were 2 µM Hsp90 (Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).
3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).
4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.
Purity
>90% SDS-PAGE.ab80033 is Affinity Purified, Endotoxin-freeExpression system
Baculovirus infected BTI-TN-5B1-4 cellsAccession
P08238Protein length
Full length proteinAnimal free
NoNature
RecombinantSpecies
HumanSequence
MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDA LDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLIN NLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHN DDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKE VVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKI EDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE YGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKK NNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKI LKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRR LSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFV ERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEK KKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTA NMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKA VKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEP NAAVPDEIPPLEGDEDASRMEEVDAmino acids
1 to 724
Associated products
Related Products
- Anti-Hsp90 beta antibody [H90-10] (ab53497)
Specifications
Our Abpromise guarantee covers the use ofab80033in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Western blot
ELISA
Inhibition Assay
Functional Studies
SDS-PAGE
Form
LiquidAdditional notes
ab80033 is endotoxin free. <0.1>
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.0154% DTT, 0.242% Tris, 0.00292% EDTA, 10% Glycerol (glycerin, glycerine), 1.015% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Alternative names
- 90 kda heat shock protein beta HSP90 beta
- D6S182
- FLJ26984
see allFunction
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.Sequence similarities
Belongs to the heat shock protein 90 family.Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.Post-translationalmodifications
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).ISGylated.S-nitrosylated; negatively regulates the ATPase activity.Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.- Information by UniProt
