Abcam/重组人EGF蛋白（无动物）（ab9697）/ab9697
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美元价：
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联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
Product name
Recombinant human EGF protein (Animal Free)See all EGF proteins and peptidesBiological activity
The ED50,as determined by a cell proliferation assay using balb/c 3T3 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Purity
>= 98% SDS-PAGE.Sterile filtered.Greater than 98% pure by HPLC analyses.Endotoxin level
<>Expression system
Escherichia coliAccession
Q6QBS2Protein length
Full length proteinAnimal free
YesNature
RecombinantSpecies
HumanSequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELRPredicted molecular weight
6 kDaAmino acids
971 to 1023Additional sequence information
ab9697 is a globular protein containing 53 amino acid residues, including 2 intramolecular disulfide bonds.
Associated products
Related Products
- Anti-EGF antibody (ab9695)
Specifications
Our Abpromise guarantee covers the use ofab9697in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Western blot
Cellular Activation
SDS-PAGE
HPLC
Form
LyophilizedAdditional notes
Manufactured using all non-animal reagents.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 500ul sterile filtered water to a concentration of 1mg/ml.
General Info
Alternative names
- Beta urogastrone
- beta-urogastrone
- EGF
see allFunction
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.Involvement in disease
Hypomagnesemia 4Sequence similarities
Contains 9 EGF-like domains.Contains 9 LDL-receptor class B repeats.Post-translationalmodifications
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).Cellular localization
Membrane.- Information by UniProt
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
