Overview
Product name
Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets)See all Cytokine Antibody Array antibody arraysSample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture mediaAssay type
Semi-quantitativeSpecies reactivity
Reacts with:HumanProduct overview
ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.
Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair ofspots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
Notes
A table listing all of ourhuman membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applications
Suitable for:Multiplex Protein Detectionmore details
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti-Cytokines 1 x 2 vials 1 x 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Angiogenesis Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Research areas
- Immunology
- Innate Immunity
- Cytokines
- Interleukins
- Immunology
- Innate Immunity
- Cytokines
- Interferons
- Antibody Arrays
- Membrane antibody arrays
- Cytokine antibody arrays
