Overview
Product name
Human MMP Antibody Array - Membrane (10 Targets)See all MMP Antibody Array antibody arraysSample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture mediaAssay type
Semi-quantitativeSpecies reactivity
Reacts with:HumanProduct overview
ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.
Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Antibodyarrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP.The antibodyarray is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
Notes
If you are interested in this antibodyarray, arrayab169816 may also be of interest.
A table of human antibody membrane arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applications
Suitable for:Multiplex Protein Detectionmore details
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 2 x 25ml Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Research areas
- Cardiovascular
- Angiogenesis
- Adhesion / ECM
- Matrix Metalloproteinases
- TIMP
- Cardiovascular
- Angiogenesis
- Adhesion / ECM
- Matrix Metalloproteinases
- MMP
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs
- Antibody Arrays
- Membrane antibody arrays
- EGFR phosphorylation antibody arrays
- Antibody Arrays
- Membrane antibody arrays
- Matrix metalloproteinase antibody arrays
