Overview
Product name
FirePlex® miRNA Assay Core Reagent Kit V2 - FFPE and TissuesProduct overview
The FirePlex®miRNA Assay enables high-throughput detection by flow cytometry of up to 65 microRNA targets and streamlined analysis with included FirePlex®Analysis Workbench software.
Our multiplex miRNA assays use our FirePlex®particle technology to profile up to 65 miRNA directly from 5 micron sections of FFPE or fresh-frozen tissue, without the need for RNA purification. The assay provides PCR sensitivity while eliminating the need for separate reverse transcription reactions and mitigating amplification biases introduced by target-specific qPCR. This is made possible by combining uniquely encoded FirePlex®hydrogel particles with single-step RT-PCR signal amplification using universal primers. The entire assay is performed in 96-well plate format such that users detect up to 65 microRNA targets for each of 96 samples in less than a day’s work.
The FirePlex®miRNA Assay reliably detects as few as 1000 microRNA copies per sample with a linear dynamic range of ~5 logs. In addition to increased sensitivity, the assay"s multiplexed detection conserves precious sample by detecting multiple microRNA targets from only 5 micron of tissue sample.
For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit https://www.abcam.com/nav/multiplex-assays/fireplex-mirna-assays
Notes
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
Properties
Storage instructions
Please refer to protocols.Components 1 x 96 tests 10x Rinse Buffer A 1 x 30ml 1x Hyb Buffer (Hybridization Buffer) 1 x 25ml 2x Rinse Buffer B 1 x 33ml 50x Labeling Buffer 1 x 200µl 5x Reporter Solution 1 x 4ml Catch Plate 2 units Digest Buffer 1 x 30ml dNTP mix 1 x 120µl FFPE/Tissue Control 1 unit Filter Plate 2 units Labeling Diluent 1 x 30ml Labeling Enzyme 1 x 50µl PCR Buffer 1 x 2ml PCR Enzyme 1 x 60µl Primer Mix 1 x 250µl Protease Mix 1 x 2ml RNA Control 1 x 50µl
