Overview
Product name
FirePlex miRNA Panel- Pancreatic ToxicityDetection method
Flow cytometry-fluorescentAssay type
MultiplexAssay time
4h00mSpecies reactivity
Reacts with:Rat, DogProduct overview
Multiplex miRNA Assay - Pancreatic Toxicity Panel (ab219510) are the FirePlex® particles designed to profile32 literature curated miRNA for pancreatic injury and toxicity, miRNAs, selected based on peer-reviewed publications modeling drug-induced pancreatic toxicity and injury. Included in this panel are miRNAs with pancreas-specific or pancreas-enriched expression, miRNAs identified as circulating biomarkers of pancreatic toxicity and internal controls to validate assay performance.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
Notes
To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Rno = Rattus norvegicus (rat), Cfa = Canis familiaris (dog), Ath = Arabidopsis thaliana (arabidposis), Oan = Ornithorhynchus anatinus (platypus), Cel = Caenorhabditis elegans (worm).
#
miRNA 21 name
miRNA Sequence (miRBase V21)
MIMAT ID
1
rno-miR-216a
UAAUCUCAGCUGGCAACUGUGA
MIMAT0000886
2
cfa-miR-216a
UAAUCUCAGCUGGCAACUGUG
MIMAT0009849
3
rno-miR-216b-5p
AAAUCUCUGCAGGCAAAUGUGA
MIMAT0017846
4
rno-miR-217
UACUGCAUCAGGAACUGACUGG
MIMAT0000887
5
cfa-miR-217
AUACUGCAUCAGGAACUGAUU
MIMAT0009850
6
rno-miR-375-3p
UUUGUUCGUUCGGCUCGCGUGA
MIMAT0005307
7
rno-miR-148a-3p
UCAGUGCACUACAGAACUUUG
MIMAT0035725
8
rno-miR-148b-3p
UCAGUGCAUCACAGAACUUUGU
MIMAT0000579
9
rno-miR-320-3p
AAAAGCUGGGUUGAGAGGGCGA
MIMAT0000903
10
rno-miR-152-3p
UCAGUGCAUGACAGAACUUGG
MIMAT0000854
11
rno-mir-92a-3p
UAUUGCACUUGUCCCGGCCUG
MIMAT0000816
12
rno-miR-96-5p
UUUGGCACUAGCACAUUUUUGCU
MIMAT0000818
13
rno-miR-129-5p
CUUUUUGCGGUCUGGGCUUGC
MIMAT0000600
14
rno-miR-133b
UUUGGUCCCCUUCAACCAGCUA
MIMAT0003126
15
rno-miR-139-5p
UCUACAGUGCACGUGUCUCCAG
MIMAT0000845
16
rno-mir-188-5p
CAUCCCUUGCAUGGUGGAGGG
MIMAT0005301
17
rno-miR-204
UUCCCUUUGUCAUCCUAUGCCU
MIMAT0000877
18
rno-miR-299a-5p
UGGUUUACCGUCCCACAUACAU
MIMAT0000901
19
rno-miR-337-3p
UUCAGCUCCUAUAUGAUGCCUUU
MIMAT0000577
20
cfa-mir-371
ACUCAAAAAAUGGCGGCACUUU
MIMAT0007747
21
rno-miR-383
CAGAUCAGAAGGUGACUGUGG
MIMAT0003114
22
rno-mir-101a-3p
UACAGUACUGUGAUAACUGAA
MIMAT0000823
23
rno-miR-141-3p
UAACACUGUCUGGUAAAGAUGG
MIMAT0000846
24
cfa-miR-141
AACACUGUCUGGUAAAGAUGG
MIMAT0009876
25
rno-miR-193b-3p
AACUGGCCCACAAAGUCCC
MIMAT0035734
26
rno-miR-200c-3p
UAAUACUGCCGGGUAAUGAUG
MIMAT0000873
27
rno-miR-217-5p
UACUGCAUCAGGAACUGACUGG
MIMAT0000887
28
rno-miR-16-5p
UAGCAGCACGUAAAUAUUGGCG
MIMAT0000785
29
rno-miR-192-5p
CUGACCUAUGAAUUGACAGCC
MIMAT0000867
30
rno-miR-29a
UAGCACCAUCUGAAAUCGGUUA
MIMAT0000802
31
rno-miR-186
CAAAGAAUUCUCCUUUUGGGCU
MIMAT0000863
32
rno-miR-122
UGGAGUGUGACAAUGGUGUUUG
MIMAT0000827
33
ath-mir167d
UGAAGCUGCCAGCAUGAUCUGG
MIMAT0000905
34
cel-mir-70-3p
UAAUACGUCGUUGGUGUUUCCAU
MIMAT0000042
35
oan-miR-7417-5p
UUCCCCACUCUGAGCACACAGC
MIMAT0028982
36
x-control
x-control
37
blank
blank
Platform
Multiplex
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 48 tests 1 x 96 tests FirePlex®Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
