Overview
Product name
FirePlex miRNA Panel- Cardiac ToxicityDetection method
Flow cytometry-fluorescentAssay type
MultiplexAssay time
4h00mSpecies reactivity
Reacts with:Rat, DogProduct overview
Multiplex miRNA Assay - Cardiac Toxicity Panel (ab219509) are the FirePlex® particles designedto profile 28 literature curated miRNA for cardiac injury and toxicity,miRNAs, selected based on peer-reviewed publications modeling drug-induced cardiac toxicity and injury. Included in this panel are miRNAs with cardiac-specific or cardiac-enriched expression, miRNAs identified as circulating biomarkers of cardiotoxicity and internal controls to validate assay performance.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use ourFirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays, here.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
Notes
To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Rno = Rattus norvegicus (rat), Cfa = Canis familiaris (dog), Ath = Arabidopsis thaliana (arabidposis), Oan = Ornithorhynchus anatinus (platypus), Cel = Caenorhabditis elegans (worm).
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miRNA 21 name
miRNA Sequence (miRBase V21)
MIMAT ID
1
rno-miR-208a-3p
AUAAGACGAGCAAAAAGC
MIMAT0000880
2
mmu-miR-208a-3p
AUAAGACGAGCAAAAAGCUUGU
MIMAT0000520
3
rno-miR-208a-5p
GAGCUUUUGGCCCGGGUUAUAC
MIMAT0017155
4
rno-miR-126a-3p
UCGUACCGUGAGUAAUAAUGCG
MIMAT0000832
5
rno-miR-6314
AGGCACUGACAGAUCUGAUGGU
MIMAT0025047
6
rno-miR-509-3p
UGAUUGACAUGUCUGCAGUGGA
MIMAT0024850
7
rno-miR-1-3p
UGGAAUGUAAAGAAGUGUGUAU
MIMAT0003125
8
rno-miR-499-5p
UUAAGACUUGCAGUGAUGUUU
MIMAT0003381
9
rno-miR-133a-3p
UUUGGUCCCCUUCAACCAGCUG
MIMAT0000839
10
rno-miR-133b-3p
UUUGGUCCCCUUCAACCAGCUA
MIMAT0003126
11
rno-mir-92a-3p
UAUUGCACUUGUCCCGGCCUG
MIMAT0000816
12
rno-miR-96
UUUGGCACUAGCACAUUUUUGCU
MIMAT0000818
13
rno-miR-129
CUUUUUGCGGUCUGGGCUUGC
MIMAT0000600
14
rno-miR-133b
UUUGGUCCCCUUCAACCAGCUA
MIMAT0003126
15
rno-miR-139
UCUACAGUGCACGUGUCUCCAG
MIMAT0000845
16
rno-mir-188-5p
CAUCCCUUGCAUGGUGGAGGG
MIMAT0005301
17
rno-miR-204
UUCCCUUUGUCAUCCUAUGCCU
MIMAT0000877
18
rno-miR-299
UGGUUUACCGUCCCACAUACAU
MIMAT0000901
19
rno-miR-337
UUCAGCUCCUAUAUGAUGCCUUU
MIMAT0000577
20
cfa-mir-371
ACUCAAAAAAUGGCGGCACUUU
MIMAT0007747
21
rno-miR-383
CAGAUCAGAAGGUGACUGUGG
MIMAT0003114
22
rno-miR-375
UUUGUUCGUUCGGCUCGCGUGA
MIMAT0005307
23
rno-miR-206-3p
UGGAAUGUAAGGAAGUGUGUGG
MIMAT0000879
24
rno-miR-16-5p
UAGCAGCACGUAAAUAUUGGCG
MIMAT0000785
25
rno-miR-192-5p
CUGACCUAUGAAUUGACAGCC
MIMAT0000867
26
rno-miR-29a
UAGCACCAUCUGAAAUCGGUUA
MIMAT0000802
27
rno-miR-186
CAAAGAAUUCUCCUUUUGGGCU
MIMAT0000863
28
rno-miR-122
UGGAGUGUGACAAUGGUGUUUG
MIMAT0000827
29
ath-mir167d
UGAAGCUGCCAGCAUGAUCUGG
MIMAT0000905
30
cel-mir-70-3p
UAAUACGUCGUUGGUGUUUCCAU
MIMAT0000042
31
oan-miR-7417-5p
UUCCCCACUCUGAGCACACAGC
MIMAT0028982
32
x-control
x-control
33
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Platform
Multiplex
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 48 tests 1 x 96 tests FirePlex®Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
