Overview
Product name
FirePlex® miRNA Panel – Cardiology V2Detection method
Flow cytometry-fluorescentAssay type
MultiplexAssay time
4h00mSpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
Multiplex miRNA Assay - Cardiology Panel (ab218368) are the FirePlex® particles designed to profile up to 65 literature curated miRNA for cardiology studies, miRNAsdifferentially regulated in normal or abnormal cardiovascular health.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technologywith the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.
These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes.
Included in the panel are general markers for multiple cardiovascular-related diseases, markers specific to specific cardiovascular states or treatment responses, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls to validate assay performance.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
Notes
To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans.
# miRNA 21 name miRNA Sequence (miRBase V21) Sequence homology ** MIMAT ID 1 hsa-let-7d-5p agagguaguagguugcauaguu Hu, Ms, Rt MIMAT0000065 2 hsa-let-7e-5p ugagguaggagguuguauaguu Hu, Ms, Rt MIMAT0000066 3 hsa-let-7g-5p ugagguaguaguuuguacaguu Hu, Ms, Rt MIMAT0000414 4 hsa-let-7i-5p ugagguaguaguuugugcuguu Hu, Ms, Rt MIMAT0000415 5 hsa-miR-1-3p uggaauguaaagaaguauguau Hu, Ms, Rt MIMAT0000416 6 hsa-miR-103a-3p agcagcauuguacagggcuauga Hu, Ms, Rt MIMAT0000101 7 hsa-miR-106b-5p uaaagugcugacagugcagau Hu, Ms, Rt MIMAT0000680 8 hsa-miR-122-5p uggagugugacaaugguguuug Hu, Ms, Rt MIMAT0000421 9 hsa-miR-124-3p uaaggcacgcggugaaugcc Hu, Ms, Rt MIMAT0000422 10 hsa-miR-125a-5p ucccugagacccuuuaaccuguga Hu, Ms, Rt MIMAT0000443 11 hsa-miR-125b-5p ucccugagacccuaacuuguga Hu, Ms, Rt MIMAT0000423 12 hsa-miR-126-3p ucguaccgugaguaauaaugcg Hu, Ms, Rt MIMAT0000445 13 hsa-miR-133a-3p uuugguccccuucaaccagcug Hu, Ms, Rt MIMAT0000427 14 hsa-miR-133b uuugguccccuucaaccagcua Hu, Ms, Rt MIMAT0000770 15 hsa-miR-142-3p uguaguguuuccuacuuuaugga Hu, Ms, Rt MIMAT0000434 16 hsa-miR-144-5p ggauaucaucauauacuguaag Hu MIMAT0004600 17 hsa-miR-145-5p guccaguuuucccaggaaucccu Hu, Ms, Rt MIMAT0000437 18 hsa-miR-146a-5p ugagaacugaauuccauggguu Hu, Ms, Rt MIMAT0000449 19 hsa-miR-150-5p ucucccaacccuuguaccagug Hu, Ms, Rt MIMAT0000451 20 hsa-miR-155-5p uuaaugcuaaucgugauaggggu Hu MIMAT0000646 21 hsa-miR-15b-5p uagcagcacaucaugguuuaca Hu, Ms, Rt MIMAT0000417 22 hsa-miR-16-5p uagcagcacguaaauauuggcg Hu, Ms, Rt MIMAT0000069 23 hsa-miR-17-5p caaagugcuuacagugcagguag Hu, Ms, Rt MIMAT0000070 24 hsa-miR-181b-5p aacauucauugcugucggugggu Hu, Ms, Rt MIMAT0000257 25 hsa-miR-18a-5p uaaggugcaucuagugcagauag Hu, Ms, Rt MIMAT0000072 26 hsa-miR-192-5p cugaccuaugaauugacagcc Hu, Ms, Rt MIMAT0000222 27 hsa-miR-194-5p uguaacagcaacuccaugugga Hu, Ms, Rt MIMAT0000460 28 hsa-miR-195-5p uagcagcacagaaauauuggc Hu, Ms, Rt MIMAT0000461 29 hsa-miR-199a-3p acaguagucugcacauugguua Hu, Ms, Rt MIMAT0000232 30 hsa-miR-199a-5p cccaguguucagacuaccuguuc Hu, Ms, Rt MIMAT0000231 31 hsa-miR-19a-3p ugugcaaaucuaugcaaaacuga Hu, Ms, Rt MIMAT0000073 32 hsa-miR-208a-3p auaagacgagcaaaaagcuugu Hu, Ms MIMAT0000241 33 hsa-miR-208b-3p auaagacgaacaaaagguuugu Hu, Ms MIMAT0004960 34 hsa-miR-20b-5p caaagugcucauagugcagguag Hu, Ms, Rt MIMAT0001413 35 hsa-miR-210-3p cugugcgugugacagcggcuga Hu, Ms, Rt MIMAT0000267 36 hsa-miR-214-3p acagcaggcacagacaggcagu Hu, Ms MIMAT0000271 37 hsa-miR-21-5p uagcuuaucagacugauguuga Hu, Ms, Rt MIMAT0000076 38 hsa-miR-25-3p cauugcacuugucucggucuga Hu, Ms, Rt MIMAT0000081 39 hsa-miR-26a-5p uucaaguaauccaggauaggcu Hu, Ms, Rt MIMAT0000082 40 hsa-miR-26b-5p uucaaguaauucaggauaggu Hu, Ms, Rt MIMAT0000083 41 hsa-miR-27a-3p uucacaguggcuaaguuccgc Hu, Ms, Rt MIMAT0000084 42 hsa-miR-27b-3p uucacaguggcuaaguucugc Hu, Ms, Rt MIMAT0000419 43 hsa-miR-28-5p aaggagcucacagucuauugag Hu, Ms, Rt MIMAT0000085 44 hsa-miR-29a-3p uagcaccaucugaaaucgguua Hu, Ms, Rt MIMAT0000086 45 hsa-miR-29b-3p uagcaccauuugaaaucaguguu Hu, Ms, Rt MIMAT0000100 46 hsa-miR-30a-5p uguaaacauccucgacuggaag Hu, Ms, Rt MIMAT0000087 47 ath-mir167d ugaagcugccagcaugaucugg Ath MIMAT0000905 48 hsa-miR-320b aaaagcuggguugagagggcaa Hu MIMAT0005792 49 hsa-miR-328-3p cuggcccucucugcccuuccgu Hu, Ms, Rt MIMAT0000752 50 hsa-miR-335-5p ucaagagcaauaacgaaaaaugu Hu, Ms, Rt MIMAT0000765 51 hsa-miR-337-5p gaacggcuucauacaggaguu Hu MIMAT0004695 52 hsa-miR-342-3p ucucacacagaaaucgcacccgu Hu, Ms, Rt MIMAT0000753 53 hsa-miR-34a-5p uggcagugucuuagcugguugu Hu, Ms, Rt MIMAT0000255 54 hsa-miR-363-3p aauugcacgguauccaucugua Hu MIMAT0000707 55 hsa-miR-370-3p gccugcugggguggaaccuggu Hu, Ms, Rt MIMAT0000722 56 oan-miR-7417-5p uuccccacucugagcacacagc Oan MIMAT0028982 57 hsa-miR-375 uuuguucguucggcucgcguga Hu, Ms, Rt MIMAT0000728 58 cel-mir-70-3p uaauacgucguugguguuuccau Cel MIMAT0000042 59 hsa-miR-423-5p ugaggggcagagagcgagacuuu Hu, Ms MIMAT0004748 60 hsa-miR-433-3p aucaugaugggcuccucggugu Hu, Ms, Rt MIMAT0001627 61 hsa-miR-451a aaaccguuaccauuacugaguu Hu, Ms, Rt MIMAT0001631 62 hsa-miR-485-3p gucauacacggcucuccucucu Hu MIMAT0002176 63 hsa-miR-486-5p uccuguacugagcugccccgag Hu, Ms, Rt MIMAT0002177 64 hsa-miR-499a-5p uuaagacuugcagugauguuu Hu, Ms, Rt MIMAT0002870 65 hsa-miR-505-5p gggagccaggaaguauugaugu Hu MIMAT0004776 66 hsa-miR-590-5p gagcuuauucauaaaagugcag Hu, Ms MIMAT0003258 67 hsa-miR-92a-3p uauugcacuugucccggccugu Hu MIMAT0000092 68 hsa-miR-93-5p caaagugcuguucgugcagguag Hu, Ms, Rt MIMAT0000093 69 x-control x-control N/A 70 blank blank N/A Platform
Multiplex
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 48 tests 1 x 96 tests FirePlex®Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
