Overview
Product name
FirePlex® miRNA Panel –Liver ToxV2Detection method
Flow cytometry-fluorescentAssay type
MultiplexAssay time
4h00mSpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
Multiplex miRNA Assay -Liver ToxPanel (ab218370) are the FirePlex® particles designed to profile up to 65 literature curated miRNA for liver toxicity.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technology with the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.
These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in whole blood, plasma, serum, peripheral blood mononuclear cells (PBMCs) or isolated exosomes.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
Notes
To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Hu = Human, Ms = Mouse, Rt = Rat, Ath = Arabidopsis thaliana, Oan = Ornithorhynchus anatinus, Cel = Caenorhabditis elegans.
# miRNA 21 name miRNA Sequence (miRBase V21) Sequence homology ** MIMAT ID 1 hsa-let-7d-5p agagguaguagguugcauaguu Hu, Ms, Rt MIMAT0000065 2 hsa-let-7g-5p ugagguaguaguuuguacaguu Hu, Ms, Rt MIMAT0000414 3 hsa-let-7i-5p ugagguaguaguuugugcuguu Hu, Ms, Rt MIMAT0000415 4 hsa-miR-100-5p aacccguagauccgaacuugug Hu, Ms, Rt MIMAT0000098 5 hsa-miR-101-3p uacaguacugugauaacugaa Hu, Ms, Rt MIMAT0000099 6 hsa-miR-107 agcagcauuguacagggcuauca Hu, Ms, Rt MIMAT0000104 7 hsa-miR-122-5p uggagugugacaaugguguuug Hu, Ms, Rt MIMAT0000421 8 hsa-miR-124-3p uaaggcacgcggugaaugcc Hu, Ms, Rt MIMAT0000422 9 hsa-miR-125b-5p ucccugagacccuaacuuguga Hu, Ms, Rt MIMAT0000423 10 hsa-miR-130a-3p cagugcaauguuaaaagggcau Hu, Ms, Rt MIMAT0000425 11 hsa-miR-146a-5p ugagaacugaauuccauggguu Hu, Ms, Rt MIMAT0000449 12 hsa-miR-146b-5p ugagaacugaauuccauaggcu Hu, Ms MIMAT0002809 13 hsa-miR-148a-3p ucagugcacuacagaacuuugu Hu, Ms MIMAT0000243 14 hsa-miR-152-3p ucagugcaugacagaacuugg Hu, Ms, Rt MIMAT0000438 15 mmu-miR-155-5p uuaaugcuaauugugauaggggu Ms, Rt MIMAT0000165 16 hsa-miR-15a-5p uagcagcacauaaugguuugug Hu, Ms MIMAT0000068 17 hsa-miR-16-5p uagcagcacguaaauauuggcg Hu, Ms, Rt MIMAT0000069 18 hsa-miR-17-5p caaagugcuuacagugcagguag Hu, Ms, Rt MIMAT0000070 19 hsa-miR-181a-5p aacauucaacgcugucggugagu Hu, Ms, Rt MIMAT0000256 20 mmu-miR-182-5p uuuggcaaugguagaacucacaccg Ms, Rt MIMAT0000211 21 hsa-miR-183-3p gugaauuaccgaagggccauaa Hu, Ms MIMAT0004560 22 hsa-miR-183-5p uauggcacugguagaauucacu Hu, Ms, Rt MIMAT0000261 23 hsa-miR-185-5p uggagagaaaggcaguuccuga Hu, Ms, Rt MIMAT0000455 24 hsa-miR-18a-5p uaaggugcaucuagugcagauag Hu, Ms, Rt MIMAT0000072 25 hsa-miR-192-5p cugaccuaugaauugacagcc Hu, Ms, Rt MIMAT0000222 26 hsa-miR-193a-3p aacuggccuacaaagucccagu Hu, Ms, Rt MIMAT0000459 27 hsa-miR-194-5p uguaacagcaacuccaugugga Hu, Ms, Rt MIMAT0000460 28 hsa-miR-199a-3p acaguagucugcacauugguua Hu, Ms, Rt MIMAT0000232 29 hsa-miR-199a-5p cccaguguucagacuaccuguuc Hu, Ms, Rt MIMAT0000231 30 hsa-miR-19a-3p ugugcaaaucuaugcaaaacuga Hu, Ms, Rt MIMAT0000073 31 hsa-miR-200a-3p uaacacugucugguaacgaugu Hu, Ms, Rt MIMAT0000682 32 hsa-miR-200b-3p uaauacugccugguaaugauga Hu, Ms MIMAT0000318 33 hsa-miR-206 uggaauguaaggaagugugugg Hu, Ms, Rt MIMAT0000462 34 hsa-miR-210-3p cugugcgugugacagcggcuga Hu, Ms, Rt MIMAT0000267 35 hsa-miR-214-3p acagcaggcacagacaggcagu Hu, Ms MIMAT0000271 36 hsa-miR-21-5p uagcuuaucagacugauguuga Hu, Ms, Rt MIMAT0000076 37 hsa-miR-221-3p agcuacauugucugcuggguuuc Hu, Ms, Rt MIMAT0000278 38 hsa-miR-222-3p agcuacaucuggcuacugggu Hu, Ms, Rt MIMAT0000279 39 hsa-miR-223-3p ugucaguuugucaaauacccca Hu, Ms MIMAT0000280 40 hsa-miR-22-3p aagcugccaguugaagaacugu Hu, Ms, Rt MIMAT0000077 41 hsa-miR-23b-3p aucacauugccagggauuacc Hu, Ms, Rt MIMAT0000418 42 hsa-miR-24-3p uggcucaguucagcaggaacag Hu, Ms, Rt MIMAT0000080 43 hsa-miR-26b-5p uucaaguaauucaggauaggu Hu, Ms, Rt MIMAT0000083 44 hsa-miR-27b-3p uucacaguggcuaaguucugc Hu, Ms, Rt MIMAT0000419 45 hsa-miR-29a-3p uagcaccaucugaaaucgguua Hu, Ms, Rt MIMAT0000086 46 hsa-miR-29b-3p uagcaccauuugaaaucaguguu Hu, Ms, Rt MIMAT0000100 47 hsa-miR-29c-3p uagcaccauuugaaaucgguua Hu, Ms, Rt MIMAT0000681 48 hsa-miR-30a-5p uguaaacauccucgacuggaag Hu, Ms, Rt MIMAT0000087 49 hsa-miR-30c-5p uguaaacauccuacacucucagc Hu, Ms, Rt MIMAT0000244 50 hsa-miR-320a aaaagcuggguugagagggcga Hu, Ms, Rt MIMAT0000510 51 rno-miR-327 ccuugaggggcaugagggu Rt MIMAT0000561 52 hsa-miR-34a-5p uggcagugucuuagcugguugu Hu, Ms, Rt MIMAT0000255 53 mmu-miR-34b-5p aggcaguguaauuagcugauugu Ms, Rt MIMAT0000382 54 hsa-miR-34c-5p aggcaguguaguuagcugauugc Hu, Ms, Rt MIMAT0000686 55 hsa-mir-370-3p gccugcugggguggaaccuggu Hu, Ms, Rt MIMAT0000722 56 hsa-miR-375 uuuguucguucggcucgcguga Hu, Ms, Rt MIMAT0000728 57 hsa-miR-378a-3p acuggacuuggagucagaaggc Hu MIMAT0000732 58 hsa-miR-410-3p aauauaacacagauggccugu Hu, Ms, Rt MIMAT0002171 59 hsa-miR-451a aaaccguuaccauuacugaguu Hu, Ms, Rt MIMAT0001631 60 mmu-miR-503-5p uagcagcgggaacaguacugcag Ms, Rt MIMAT0003188 61 hsa-miR-652-3p aauggcgccacuaggguugug Hu, Ms, Rt MIMAT0003322 62 hsa-miR-877-5p guagaggagauggcgcaggg Hu, Ms, Rt MIMAT0004949 63 hsa-miR-92a-3p uauugcacuugucccggccugu Hu MIMAT0000092 64 hsa-miR-96-5p uuuggcacuagcacauuuuugcu Hu, Ms, Rt MIMAT0000095 65 hsa-miR-99a-5p aacccguagauccgaucuugug Hu, Ms, Rt MIMAT0000097 66 ath-mir167d ugaagcugccagcaugaucugg Ath MIMAT0000905 67 cel-mir-70-3p uaauacgucguugguguuuccau Cel MIMAT0000042 68 oan-miR-7417-5p uuccccacucugagcacacagc Oan MIMAT0028982 69 x-control 70 blank Platform
Multiplex
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 48 tests 1 x 96 tests FirePlex®Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
