Overview
Product name
HeLa Cell Fractions (Cytoplasmic/Membrane/Nuclear)Sample type
Cell culture extractsSpecies reactivity
Reacts with:HumanProduct overview
HeLa fractions were prepared using the Cell Fractionation Kit Standard (ab109719)/Membrane Fractionation Kit (ab139409). Using this kit, cells were fractionated into a cytosolic fraction, a membrane (mitochondrial-containing) fraction and a nuclear fraction without any material loss.
Notes
The cytosolic fraction contains cytosolic proteins and is mostly devoid of mitochondrial, ER, plasma membrane, and nuclear proteins. The membrane fraction contains mitochondrial, ER, and plasma membrane proteins, and is mostly devoid of cytosolic and nuclear proteins. The nuclear fraction contains mainly nuclear proteins and is devoid of cytosolic, mitochondrial, ER, and plasma membrane proteins. Each fraction is proportional to the others, allowing the user to analyze equal volumes of each fraction to determine a distribution of a target of interest between these three fractions. The fractions were lysed in SDS sample buffer containing DTT.
Tested applications
Suitable for:WB, SDS-PAGEmore details
Properties
Storage instructions
Store at -80°C. Please refer to protocols.Components 3 units HeLa Cytosolic Fraction Lysate 1 x 200µl HeLa Membrane Fraction Lysate 1 x 200µl HeLa Nuclear Fraction Lysate 1 x 200µl Research areas
- Kits/ Lysates/ Other
- Lysates
- Whole Cell Lysates
- Human
- HeLa
