Overview
Product name
HeLa Cell Cycle Lysates: Thymidine-Treated, Nocodazole-Treated & Untreated Asynchronous ControlSpecies reactivity
Reacts with:HumanProduct overview
ab136811contains 3 vials of HeLa whole cell lysates from cells in different stages of the cell cycle: asynchronous, G1/S and G2/M. Asynchronous cells were harvested from untreated, subconfluent plates and this lysate contains cells in all stages of the cell cycle. G1/S cells were harvested following a double thymidine block procedure and represents cells at the G1 to S-phase transition (excess thymidine inhibits DNA synthesis). G2/M cells were harvested following sequential treatments with thymidine and nocodazole and represents cells that have duplicated DNA but are blocked at mitosis (nocodazole inhibits microtubule polymerization).
Concentration: Untreated Lysate - 150 µg at0.5mg/mLThymidine Treated Lysate - 150 µg at 0.5 mg/mL NocodazoleTreated Lysate - 150 µg at 0.5 mg/mL
Notes
Each whole cell lysate was made by solubilizing cells in an SDS lysis buffer followed by BCA protein quantification and normalization to 0.5 mg/mL in SDS-PAGE sample buffer. A parallel treated sample of cells for each condition was analyzed by propidium iodide staining and flow cytometry to confirm cell cycle stage.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at -80°C. Please refer to protocols.Components 3 units Control for Thymidine/Nocodazole HeLa Lysates 1 x 150µg Thymidine-Treated HeLa Lysate 1 x 150µg Nocodazole-Treated HeLa Lysate 1 x 150µg Research areas
- Kits/ Lysates/ Other
- Lysates
- Whole Cell Lysates
- Human
- HeLa
