Overview
Product name
Human TSPO (PBR) knockout HeLa cell lysateSee all PBR kitsProduct overview
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.See here for more information on knockout cell lysates.User storage instructions:After reconstitution, store the lysate at -80°C.
Parental Cell Line
HeLaOrganism
HumanMutation description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2.Passage number
<>Knockout validation
Sanger Sequencing, Western Blot (WB)Reconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at -80°C. Please refer to protocols.Components 1 kit Human TSPO knockout HeLa cell lysate (Lyophilized) 1 x 100µg Human Wild Type HeLa cell lysate (Lyophilized) 1 x 100µg Research areas
- Cardiovascular
- Lipids / Lipoproteins
- Lipid Metabolism
- Cholesterol Metabolism
- Signal Transduction
- Signaling Pathway
- Calcium Signaling
- Calcium Channels
- Cell Biology
- Apoptosis
- Mitochondrial
- Signal Transduction
- Metabolism
- Mitochondrial
- Signal Transduction
- Metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolism processes
- Apoptosis
- Cancer
- Cell Death
- Apoptosis
- Mitochondrial
- Cancer
- Cell Death
- Apoptosis
- Metabolism
Cell type
epithelialDisease
AdenocarcinomaGender
FemaleSTR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Target
Function
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.Tissue specificity
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.Sequence similarities
Belongs to the TspO/BZRP family.Cellular localization
Mitochondrion membrane.- Information by UniProt
Alternative names
- Benzodiazapine receptor (peripheral)
- Benzodiazepine peripheral binding site
- BPBS
see all
