商品介绍
Overview
Product name
Bovine Heart MitochondriaGeneral notes
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam"s suggested protocols.
Tested applications
Suitable for:IP, SDS-PAGE, WBmore details
Properties
Mycoplasma free
YesForm
LiquidStorage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.Storage buffer
Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
Concentration information loading...Lysate notes
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam"s suggested protocols.
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
