Overview
Product name
Fura-2 AM, Ca2+ selective fluorescent indicatorDescription
High affinity Ca2+ selective fluorescent indicatorAlternative names
- Fura 2 acetoxymethyl ester
Biological description
High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.
Purity
>95%General notes
1 unit = 50μg
- 1 mg - 1 x 1 mg vial
- 20 units - 20 x 50 µg vials
- 10 units - 10 x 50 µg vials
- 50 µg - 1 x 50 µg vial
Related protocols:
Fura-2 AM imaging protocol
Calcium imaging using Fura-2 AM in motor neurons
Measurement of free cytosolic calcium concentration in single CHO-K1 cells
CAS Number
108964-32-5Chemical structure
Properties
Chemical name
2-[6-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl esterMolecular weight
1001.86Molecular formula
C44H47N3O24PubChem identifier
3364574Storage instructions
Shipped at Room Temperature. Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.Solubility overview
Soluble in DMSO to 10 mMHandling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
SMILES
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)CSource
Synthetic
Research areas
- Biochemicals
- Chemical Type
- Biochemicals
- Biochemicals
- Chemical Type
- Fluorescent tools
- Biochemicals
- Pharmacology
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Alzheimer"s Disease
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Diabetes
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Heart disease
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Hypertension
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Obesity
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Obesity
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Pain & inflammation
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Respiratory disease
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Stroke
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Research Area
- Stroke
- Signaling
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Kits/ Lysates/ Other
- Tools and Reagents
- Fluorescent dyes and reagents
- Biochemicals
- Research Area
- Cancer
- Ca2+ signaling
- Ionophores, Indicators & Chelators
- Biochemicals
- Chemical Type
- Dyes, stains and fluorescent tools
