Overview
Product name
Pitstop® 2, Novel cell-permeable clathrin inhibitorDescription
Novel, selective cell-permeable clathrin inhibitorBiological description
Novel, selective, cell membrane permeable clathrin inhibitor. Competitively inhibits clathrin terminal domain to selectively inhibit clathrin mediated endocytosis (CME) (IC50 = 12 μM for inhibition of amphiphysin association of clathrin TD). Interferes with receptor mediated endocytosis (RME), entry of HIV and synaptic vesicle recycling.
Purity
>98%General notes
High concentrations of Pitstop 2™ may interfere with fluorescence imaging due to a low emittance of the compound in the green channel. This fluorescence is not usually detectable if the cells have been first fixed and washed prior to imaging.
Sold under exclusive licence from Children"s Medical Research Institute and Newcastle Innovation Ltd. Pitstop® is a trademark of Freie Universitat Berlin, Newcastle Innovation Ltd. and Children"s Medical Research Institute.
CAS Number
1419093-54-1Chemical structure
Properties
Chemical name
N-[5-(4-Bromobenzylidene)-4-oxo-4,5-dihydro-1,3-thiazol-2-yl]naphthalene-1-sulfonamide
Molecular weight
473.36Molecular formula
C20H13BrN2O3S2PubChem identifier
137254289Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.Solubility overview
Soluble in DMSO. Please refer to the Protocol Booklet for more information.
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
SMILES
C1=CC=C2C(=C1)C=CC=C2S(=O)(=O)N=C3NC(=O)/C(=C/C4=CC=C(C=C4)Br)/S3Source
Synthetic
Research areas
- Biochemicals
- Chemical Type
- Biochemicals
- Biochemicals
- Pharmacology
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Heart disease
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Pain & inflammation
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Alzheimer"s Disease
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Obesity
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Respiratory disease
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Research Area
- Stroke
- Signaling
- Endocytosis & exocytosis
- Clathrin
- Biochemicals
- Pharmacology
- Signaling
- Immunomodulators
- Antiviral
