Overview
Product name
PRX Pathway (TRX, TXNRD1, PRX1) Western Blot CocktailSample type
Cell LysateSpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
The PRX pathway Western blot cocktail is designed to determine the relative abundance of several important proteins involved in the thioredoxin redox pathway.
Thioredoxin (TRX) is a small enzyme (12kDa) that facilitates the reduction of other enzymes via cysteine thiol-disulfide exchange. Thioredoxin is used by cells to reduce ROS amounts and in redox signaling processes. Thioredoxin reductase 1 (TXNRD1) is found in the cytoplasm and possesses glutaredoxin and thioredoxin reductase activity. TXNRD1 utilizes its redox-active disulfide bond to reduce oxidized thioredoxin. Also involved in the thioredoxin redox pathway and additionally shown to be upregulated in a variety of cancers is the relatively abundant peroxiredoxin 1 (PRX1). Peroxiredoxin 1 is an antioxidant enzyme that helps regulate peroxide levels and is involved in redox signaling. Peroxiredoxin 1’s activity is regenerated after being reduced by thioredoxin.
Notes
These three readouts are easily resolved by Western blot given their different molecular weights. As all primary antibodies in the WB cocktail are rabbit antibodies, an anti-rabbit secondary should be used for detection.
Expected and observed MWs:Thioredoxin: 12 kDaThioredoxin Reductase 1: 55 kDaPeroxiredoxin 1: 22 kDa
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 200 µl 250X PRX Pathway (TRX, TXNRD1, PRX1) Western Blot Cocktail 1 x 200µl Research areas
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- PRX
Database links
- Entrez Gene:5052 Human
- Entrez Gene:7295 Human
- Entrez Gene:7296 Human
- Entrez Gene:18477 Mouse
- Entrez Gene:50493 Mouse
- Entrez Gene:117254 Rat
- Entrez Gene:58819 Rat
- SwissProt:P10599 Human
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