Overview
Product name
Complex II WB Antibody CocktailSee all Complex II kitsAssay type
QuantitativeSpecies reactivity
Reacts with:Mouse, Rat, Cow, HumanProduct overview
Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Cocktail Antibodies:
Mouse C-V-alpha [15H4C4] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal:
Amount: 250 µg
Working concentration: 5 µg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:
Amount: 5 µg
Working concentration: 0.1 µg/ml
Notes
Related products
Review themitochondrial assay guide, or the fullmetabolism assay guideto learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 305 µg Pre-mixed solution of 3 monoclonal antibodies 1 x 305µg Research areas
- Signal Transduction
- Metabolism
- Mitochondrial
- Kits/ Lysates/ Other
- Kits
- Cell Metabolism Kits
- Other Metabolism Assay
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- OXPHOS
Relevance
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.Alternative names
- SDH
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
Database links
- Entrez Gene:6390 Human
- Entrez Gene:6389 Human
- Entrez Gene:6391 Human
- SwissProt:P31040 Human
- SwissProt:P21912 Human
- SwissProt:Q99643 Human
- SwissProt:O14521 Human
- SwissProt:6392 Human
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