Overview
Product name
Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa)See all Prestained Protein Ladder reagentsDescription
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)Tested applications
Suitable for:WB, SDS-PAGEmore detailsGeneral notes
Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Do not heat, dilute, add reducing agent before loading.
Key product features:
- Broad range: 10-180 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~2 - 3 µl for western blots
Review other protein ladders in theunstained and prestained protein ladder guide.
Properties
Form
LiquidStorage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.Storage buffer
pH: 7.5Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol
Concentration information loading...Research areas
- Kits/ Lysates/ Other
- Tools and Reagents
- Western Blot Tools and Reagents
- Protein ladders
Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all
