Overview
Product name
Cell Cycle (pCdk/pHH3/Actin) WB CocktailSpecies reactivity
Reacts with:Mouse, HumanProduct overview
The Cell Cycle western blot cocktail (ab136810) is designed to provide a rapid assessment of cell cycle distribution based on molecular markers for the G1/S and M phases of the cell cycle. This is a mixture of three specific rabbit monoclonal primary antibodies targeting phospho-cdk2 Tyr15, phospho Histone H3 Ser10 and beta-actin. Cyclin-dependent kinase 2 (Cdk2) is maintained in an inactive state in G1/S by inhibitory phosphorylation on Tyr15. Histone H3 is phosphorylated at Ser10 when chromosomes condense during mitosis. Hence, elevated phospho-cdk2 Tyr15 indicates G1/S arrested cells and elevated phospho Histone H3 Ser10 indicates M-phase arrested cells when compared to asynchronous cycling control cells. An anti- Actin antibody is included as a loading control. These three readouts are easily resolved by western blot given their different molecular weights.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 200 µl 250X Cell Cycle WB Cocktail 1 x 200µl Research areas
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- Apoptosis & Cell Cycle
