Overview
Product name
Prestained Protein Ladder – Broad molecular weight (10-245 kDa)See all Prestained Protein Ladder reagentsDescription
Prestained Protein Ladder - Broad molecular weight (10-245 kDa)Tested applications
Suitable for:SDS-PAGE, WBmore detailsGeneral notes
Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).
This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.
Key product features:
- Broad range: 10-245 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
- Sharp bands
- Recommended loading: ~1.5 - 2.5µl
Review other protein ladders in theunstained and prestained protein ladder guide.
This product was previously calledPrism Ultra Protein Ladder (10-245 kDa).
Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Properties
Form
LiquidStorage instructions
Please see notes section.Storage buffer
pH: 7.5Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol- Concentration information loading...
Research areas
- Kits/ Lysates/ Other
- Tools and Reagents
- Western Blot Tools and Reagents
- Protein ladders
Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all