Abcam/Native lysis Buffer (ab156035)/1/ab156035
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3392242852
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4000-520-616
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info@ebiomall.com
商品介绍
Overview
Product name
Native lysis BufferSee all Lysis Buffer reagentsTested applications
Suitable for:WB, Sandwich ELISA, SDS-PAGE, IPmore detailsGeneral notes
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.This reagent extracts cytoplasmic, nuclear and membrane proteins.The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity.On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g. ab156034). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Properties
Form
LiquidStorage instructions
Store at +4°C.Storage buffer
pH: 7.50Constituents: 0.02% Beta glycerophosphate, 0.02% Sodium orthovanadate, 0.04% EGTA, 0.6% HEPES, 0.03% EDTA, 0.58% Sodium chloride, 1.5% Dodecyl maltoside, 0.11% Sodium pyrophosphate decahydrate
Concentration information loading...Research areas
- Kits/ Lysates/ Other
- Tools and Reagents
- Lysis
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
