Description
Product name
Recombinant human CD19 protein (Fc Chimera Active)See all CD19 proteins and peptidesBiological activity
Measured by its binding ability in a functional ELISA against immobilized FMC63mAb (0.2μg/well). The linear range is 0.15-1.25 μg/mL.
Measured by its binding ability in a functional ELISAagainst immobilized CD19-Fc at 2 µg/ml ( 100 µl/well ). The linear ranger of 0.04 - 0.75 µg/ml.
Purity
>95% SDS-PAGE.Endotoxin level
<>Expression system
HEK 293 cellsAccession
P15391Protein length
Protein fragmentAnimal free
NoNature
RecombinantSpecies
HumanSequence
PEEPLVVKVE EGDNAVLQCL KGTSDGPTQQ LTWSRESPLK PFLKLSLGLP GLGIHMRPLA IWLFIFNVSQ QMGGFYLCQP GPPSEKAWQP GWTVNVEGSG ELFRWNVSDL GGLGCGLKNR SSEGPSSPSG KLMSPKLYVW AKDRPEIWEG EPPCLPPRDS LNQSLSQDLT MAPGSTLWLS CGVPPDSVSR GPLSWTHVHP KGPKSLLSLE LKDDRPARDM WVMETGLLLP RATAQDAGKY YCHRGNLTMS FHLEITARPV LWHWLLRTGG WKPredicted molecular weight
56 kDa including tagsAmino acids
20 to 291Tags
Fc tag C-TerminusAdditional sequence information
(AAH06338)
Specifications
Our Abpromise guarantee covers the use ofab167709in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Flow Cytometry
SDS-PAGE
Functional Studies
Form
LyophilizedAdditional notes
Endotoxin levels determined by the LAL method.
After reconstitution, under sterile conditions, no activity loss observed after storage at -70°C for 3 months.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40Constituents: PBS, 5% TrehaloseLyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with sterile deionized water to a concentration of 250 µg/ml.
General Info
Alternative names
- Antibody deficiency due to defect in CD19
- Antibody deficiency due to defect in CD19, included
- AW495831
see allFunction
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Post-translationalmodifications
Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.Cellular localization
Membrane.- Information by UniProt
