商品介绍
Description
Product name
Recombinant human Hsc70 proteinSee all Hsc70 proteins and peptidesBiological activity
ab78431 has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.Expression system
Escherichia coliAccession
P11142Protein length
Full length proteinAnimal free
NoNature
RecombinantSpecies
HumanPredicted molecular weight
73 kDa including tagsAmino acids
1 to 646Tags
His tag N-TerminusAdditional sequence information
His tag
Associated products
Related Products
- Anti-Hsc70 antibody (ab1427)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Hsc70 antibody [EP1531Y] (ab51052)
Specifications
Our Abpromise guarantee covers the use ofab78431in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Western blot
ELISA
Functional Studies
competitive binding assays
SDS-PAGE
Form
Liquid
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 7.50Preservative: 1.36% ImidazoleConstituents: 0.87% Sodium chloride, 10% Glycerol, 0.328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Alternative names
- 2410008N15Rik
- Constitutive heat shock protein 70
- Epididymis luminal protein 33
see allFunction
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.Tissue specificity
Ubiquitous.Sequence similarities
Belongs to the heat shock protein 70 family.Domain
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.Post-translationalmodifications
Phosphorylated upon DNA damage, probably by ATM or ATR.ISGylated.Cellular localization
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.- Information by UniProt
品牌介绍
Abcam超过1800种高质量的纯化二抗已在多种应用中得到验证，如蛋白质印迹，免疫组织化学，免疫细胞化学，ELISA和流式细胞仪。了解Abcam如何支持您的研究。
