Description
Product name
Recombinant human TNF alpha protein (Active)See all TNF alpha proteins and peptidesBiological activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 0.71ng/mL corresponding to a Specific Activity of 1.41 x 106IU/mg.
Purity
>= 95% Immunogen affinity purified.>= 95 % HPLC.Endotoxin level
<>Expression system
HEK 293 cellsAccession
P01375Protein length
Full length proteinAnimal free
YesCarrier free
YesNature
RecombinantSpecies
HumanSequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVV PSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQV YFGIIALPredicted molecular weight
17 kDaAmino acids
77 to 233Additional sequence information
Full length mature chain soluble form. N-terminal glycine.
Associated products
Specifications
Our Abpromise guarantee covers the use ofab259410in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Cell Culture
Functional Studies
Mass Spectrometry
HPLC
SDS-PAGE
Form
LyophilizedAdditional notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with Phosphate Buffered Saline.Reconstituted protein stable at -80C for 12 months or 4C for 1 week.
General Info
Alternative names
- APC1
- APC1 protein
- Cachectin
see allFunction
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).Sequence similarities
Belongs to the tumor necrosis factor family.Post-translationalmodifications
The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.Cellular localization
Secreted and Cell membrane.- Information by UniProt
