Description
Product name
Recombinant human TNF alpha proteinSee all TNF alpha proteins and peptidesBiological activity
The ED50, as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D, is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Purity
>98% SDS-PAGE.>98% by HPLC analyses.Sterile filtered.Endotoxin level
<>Expression system
Escherichia coliAccession
P01375Protein length
Full length proteinAnimal free
NoNature
RecombinantSpecies
HumanSequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTISRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALPredicted molecular weight
17 kDaAmino acids
77 to 233Additional sequence information
aa 77 to 233 refers to the full length mature form (soluble).
Associated products
ELISA pair antibody
- Anti-TNF alpha antibody (ab9635)
Related Products
- Human TNF alpha ELISA Kit (ab181421)
- Human TNF alpha Matched Antibody Pair Kit (ab213467)
- Anti-TNF alpha antibody (ab9348)
Specifications
Our Abpromise guarantee covers the use ofab9642in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Functional Studies
Sandwich ELISA
HPLC
SDS-PAGE
Form
LyophilizedAdditional notes
Lots prior to June 2015 contain 0.036% Tris (including lots GR157466-11, -12 and -13).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.049% Sodium phosphate, 0.12% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with dH2O to make a final concentration between 0.1 to 1.0 mg/ml.
General Info
Alternative names
- APC1
- APC1 protein
- Cachectin
see allFunction
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).Sequence similarities
Belongs to the tumor necrosis factor family.Post-translationalmodifications
The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.Cellular localization
Secreted and Cell membrane.- Information by UniProt
