Description
Product name
Native human IgE protein (Azide free)See all IgE proteins and peptidesBiological activity
1 mg ofab65866 is 380,000± 50,000 IU/ml.
IgE has been used in a variety of cell stimulation assays, including the following cell types:
- Human airway smooth muscle (HASM) cells
- mast cells (MC)
- Human bronchial/tracheal smooth muscle (B/TSM) cells
- acute myelogenous leukemia (AML) blasts
- Human neutrophils
Purity
>90% SDS-PAGE.Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.Endotoxin level
<>Expression system
NativeAccession
P0DOX4P0DOX7Protein length
Full length proteinAnimal free
NoNature
NativeAmino Acid Sequence 1
Species
HumanAdditional sequence information
Observed band of ~185kDa on SDS-PAGE
Amino Acid Sequence 2
Species
Human
Associated products
Specifications
Our Abpromise guarantee covers the use ofab65866in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
SDS-PAGE
IHC - Wholemount
Western blot
ELISA
Immunocytochemistry
Form
LiquidAdditional notes
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
- Less batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25="" eu/mg="" allow="" use="" of="" ige="" protein="" in="" cellular="" immunology="" and="" in="" cell="" proliferation="">
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40Constituents: 0.00017% PBS, 0.00087% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Alternative names
- Igh2
- IGHE
- IGHEP1
- Immunoglobulin heavy constant epsilon
Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.Cellular localization
Cell Membrane
