Description
Product name
Streptavidin (HRP)See all Streptavidin proteins and peptidesBiological activity
Binds to Biotin. Dissociation constant has not been measured.
Expression system
NativeAccession
P22629Protein length
Full length proteinAnimal free
NoNature
NativeSpecies
Streptomyces avidinii
Conjugation
HRPDescription
Native Streptavidin protein (HRP)
Associated products
Substrate reagent
- ABTS™, Peroxidase substrate (ab142041)
Specifications
Our Abpromise guarantee covers the use ofab7403in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunomicroscopy
Immunocytochemistry
Western blot
Form
LiquidAdditional notes
Horseradishperoxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.
This product has been assayed against 1.0 µg of Biotinylated IgG in a standard capture ELISA using a peroxidase substrate as ABTS ab142041 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Gentamicin sulphateConstituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, BSA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Alternative names
- SA V1
- SA V2
- strepavidin
see allRelevance
Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.Cellular localization
Cytoplasmic
