Overview
Product name
Phalloidin-iFluor 488 ReagentSee all F-actin kitsSample type
Adherent cells, Suspension cellsAssay type
Cell-based (qualitative)Product overview
Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.
Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 647,Phalloidin-iFluor 594,Phalloidin-iFluor 555, andRhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
Notes
Stainingfixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Platform
Fluorescence microscope
Properties
Storage instructions
Store at -20°C. Please refer to protocols.Components 300 tests Phalloidin-iFluor 488 Conjugate 1 x 300 tests Research areas
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Microfilaments
- Actin etc
- Actin
- Kits/ Lysates/ Other
- Kits
- Cell Staining Kits
- F-actin Staining Kits
Alternative names
- actin filament
- f actin
- Filamentous actin
