Overview
Product name
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)See all ACTH kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Plasma 8 4.2% Inter-assay Sample n Mean SD CV% Plasma 3 6.6% Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
6 pg/mlRange
7.81 pg/ml -500 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 87 82%- 92% Hep Plasma 116 113%- 120% EDTA Plasma 105 100%- 110% Cit plasma 101 89%- 107% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:Mouse, RatProduct overview
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat ACTH (adrenocorticotropic hormone) with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH’s primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Mouse/Rat ACTH (adrenocorticotropic hormone) Capture Antibody 1 x 600µl 10X Mouse/Rat ACTH (adrenocorticotropic hormone) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Mouse/Rat ACTH (adrenocorticotropic hormone) Lyophilized Peptide 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml Research areas
- Neuroscience
- Neurotransmitter
- Neuropeptides
- Hormones
- Signal Transduction
- Growth Factors/Hormones
- Hormones
- Neuroscience
- Endocrine system
- Hypothalamic pituitary adrenal axis
- Cancer
- Tumor biomarkers
- Hormones
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Hormone biosynthesis
- Metabolism
- Pathways and Processes
- Endocrine metabolism
- Hormone biosynthesis
- Metabolism
- Types of disease
- Obesity
Relevance
ACTH occurs in cells of the anterior pituitary and in neurons in brain.It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.Cellular localization
SecretedAlternative names
- ACTH
- Adrenocorticotropic hormone
- Adrenocorticotropin
see allDatabase links
- Entrez Gene:18976 Mouse
- Entrez Gene:24664 Rat
- SwissProt:P01193 Mouse
- SwissProt:P01194 Rat
- Unigene:108195 Rat
