Overview
Product name
Human Clusterin ELISA KitSee all Clusterin kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 9 2.7% Inter-assay Sample n Mean SD CV% Serum 3 4.9% Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
22.5 pg/mlRange
234.4 pg/ml -15000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 100%- 106% Saliva 100 99%- 100% Milk 99 97%- 101% Urine 102 101%- 102% Serum 101 98%- 103% Cell culture extracts 105 96%- 112% Hep Plasma 102 101%- 103% EDTA Plasma 101 99%- 102% Cit plasma 107 106%- 109% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:Human, Rhesus monkeyDoes not react with:CowProduct overview
As of May 22, 2018, Human Clusterin ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.HumanClusterin ELISA kit (ab174447) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement ofClusterinprotein in human serum, plasma, urine, saliva, milk, cell culture supernatant, and cell extracts.It uses our proprietary SimpleStep ELISA® technology. Quantitate humanClusterinwith 36.1pg/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Notes
Clusterin (CLU) is composed of an antiparallel, disulfide-linked heterodimer of an alpha chain and a beta chain that self-associate and can form higher oligomers. It is ubiquitously expressed in various tissues. Clusterin functions as an extracellular chaperone that prevents aggregation of nonnative proteins. It does not require ATP and does not refold proteins by itself. Clusterin does so by maintaining partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Clusterin prevents stress-induced aggregation of blood plasma proteins by inhibiting the formation of amyloid fibrils through APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). It can bind to cell surface receptors and trigger internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Clusterin Capture Antibody 1 x 600µl 10X Human Clusterin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Clusterin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cell Biology
- Apoptosis
- Intracellular
- Associated Proteins
- Immunology
- Innate Immunity
- Complement
- Classical Pathway
- Cardiovascular
- Lipids / Lipoproteins
- Lipoproteins/Apolipoproteins
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Proteasome
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- NFkB Pathway
- Signal Transduction
- Protein Trafficking
- Chaperones
- Other Chaperones
- Epigenetics and Nuclear Signaling
- Transcription
- Transcription Factors
- Signal Transduction
- Metabolism
- Lipid metabolism
- Cancer
- Tumor biomarkers
- Other
- Cardiovascular
- Lipids / Lipoproteins
- Lipoproteins/Apolipoproteins
- Apolipoproteins
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Apoptosis marker and proteins ELISA kits
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Signal transduction proteins ELISA kits
- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- Ubiquitin Binding
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Nuclear Signaling ELISA kits
Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.Tissue specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.Sequence similarities
Belongs to the clusterin family.Post-translationalmodifications
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.Polyubiquitinated, leading to proteasomal degradation.Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.Cellular localization
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.- Information by UniProt
Alternative names
- 40
- AAG 4
- AAG4
see allDatabase links
- Entrez Gene:1191 Human
- Omim:185430 Human
- SwissProt:P10909 Human
- Unigene:436657 Human
