Overview
Product name
Mouse MCP1 ELISA KitSee all MCP1 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Mouse serum 8 4.4% Inter-assay Sample n Mean SD CV% Mouse serum 3 9.9% Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
0.487 pg/mlRange
1.56 pg/ml -100 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 91%- 107% Serum 105 101%- 112% Hep Plasma 105 103%- 108% EDTA Plasma 106 104%- 110% Cit plasma 104 100%- 111% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:MouseDoes not react with:Goat, Cow, PigProduct overview
Mouse MCP1 ELISA Kit (ab208979) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP1 with 0.487 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
MCP1 (CCL2) is a chemokine expressed by fibroblasts, macrophages, endothelial cells, mast cells, osteoblasts, ameloblasts, epithelial cells, and astrocytes after stimulation with inflammatory stimuli. MCP1 is involved in the recruitment of monocytes and lymphocytes and in the regulation of Th1/Th2 lymphocyte differentiation.
Platform
Microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Mouse MCP1 Capture Antibody 1 x 600µl 10X Mouse MCP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse MCP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Immunology
- Innate Immunity
- Chemokines
- Alpha Chemokines (CXC)
- Cardiovascular
- Atherosclerosis
- Vascular Inflammation
- Leukocyte recruitment
- Chemokines
- Cancer
- Cancer Metabolism
- Response to hypoxia
- Metabolism
- Pathways and Processes
- Metabolism processes
- Hypoxia
- Metabolism
- Types of disease
- Heart disease
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.Post-translationalmodifications
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.Cellular localization
Secreted.- Information by UniProt
Alternative names
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see allDatabase links
- Entrez Gene:20296 Mouse
- SwissProt:P10148 Mouse
- Unigene:290320 Mouse
