Overview
Product name
Human IL-6 ELISA KitSee all IL-6 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Cell Extract 5 2.1% Inter-assay Sample n Mean SD CV% Cell Extract 3 2.4% Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
1.6 pg/mlRange
7.8 pg/ml -500 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 82 77%- 84% Cell culture media 100 97%- 103% EDTA Plasma 82 80%- 85% Cit plasma 84 81%- 85% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human IL-6ELISAkit (ab178013) isa single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in human serum, plasma and cell culture supernatant.It uses our proprietary SimpleStep ELISA® technology. Quantitatehuman IL-6with 1.6 pg/mlsensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITYThis kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed. 18.Species Reactivity This kit recognizes Human IL-6 protein. Recombinant mouse IL-6 was prepared at 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Please contact our Technical Support team for more information
Notes
Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Platform
Microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human IL-6 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-6 Capture Antibody (Lyophilized) 1 vial Human IL-6 Lyophilized Recombinant Protein (ab9627) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Immunology
- Innate Immunity
- Macrophage / Inflamm.
- Immunology
- Innate Immunity
- Cytokines
- Interleukins
- Neuroscience
- Neurology process
- Metabolism
- Cancer
- Invasion/microenvironment
- Angiogenesis
- Angiogenic growth factors
- Cancer
- Tumor immunology
- Cytokines
- Interleukins
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- Kits/ Lysates/ Other
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- Metabolism
- Types of disease
- Obesity
- Metabolism
- Types of disease
- Metabolic disorders
Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.Sequence similarities
Belongs to the IL-6 superfamily.Post-translationalmodifications
N- and O-glycosylated.Cellular localization
Secreted.- Information by UniProt
Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see allDatabase links
- Entrez Gene:3569 Human
- Omim:147620 Human
- SwissProt:P05231 Human
- Unigene:654458 Human
