Overview
Product name
Human Hemopexin ELISA KitSee all Hemopexin kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 5 3.3% Inter-assay Sample n Mean SD CV% Serum 3 4.8% Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
370 pg/mlRange
0.78 ng/ml -50 ng/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 104%- 113% Serum 108 97%- 117% Hep Plasma 105 97%- 117% EDTA Plasma 94 85%- 103% Cit plasma 97 86%- 110% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanDoes not react with:CowProduct overview
Human Hemopexin ELISA Kit (ab221838) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemopexin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemopexin with 370 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer"s disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.
Platform
Microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Hemopexin Capture Antibody 1 x 600µl 10X Human Hemopexin Detector Antibody 1 x 600µl Human Hemopexin Lyophilized Native Protein 1 x 2 vials Antibody Diluent 4BI 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMBDevelopment Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit Research areas
- Signal Transduction
- Cytoskeleton / ECM
- Extracellular Matrix
- ECM Enzymes
- MMP
- Signal Transduction
- Metabolism
- Vitamins / Minerals
- Metabolism
- Pathways and Processes
- Cofactors, Vitamins / minerals
- Vitamins / minerals
Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.Tissue specificity
Expressed by the liver and secreted in plasma.Sequence similarities
Belongs to the hemopexin family.Contains 5 hemopexin-like domains.Post-translationalmodifications
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.Cellular localization
Secreted.- Information by UniProt
Alternative names
- Beta 1B glycoprotein
- Beta-1B-glycoprotein
- FLJ56652
see allDatabase links
- Entrez Gene:3263 Human
- Omim:142290 Human
- SwissProt:P02790 Human
- Unigene:426485 Human
