Overview
Product name
Human Haptoglobin ELISA KitSee all Haptoglobin kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 5 1.8% Inter-assay Sample n Mean SD CV% Serum 3 4.4% Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
86 pg/mlRange
312.5 pg/ml -20000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 97%- 113% Saliva 105 101%- 113% Urine 102 101%- 105% Serum 107 104%- 113% Hep Plasma 104 99%- 110% EDTA Plasma 105 99%- 111% Cit plasma 107 102%- 120% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanDoes not react with:CowProduct overview
Human Haptoglobin ELISA Kit (ab219048) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Haptoglobin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Haptoglobin with 86 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Haptoglobin binds free hemoglobin to allow recycling of heme iron and prevent kidney damage. The hemoglobin/haptoglobin complex is then cleared by the Kupfer cells through endocytosis, via the CD163 scavenger receptor. The precursor to haptoglobin, zonulin, is cleaved into alpha and beta chains that homo and heterodimerize to form haptoglobin. In the population, there are two major allelic forms of hemoglobin that give rise to 3 major phenotypes (1-1, 2-1, and 2-2), which are a combination of the homo and heterodimerized alpha and beta chains. This kit recognizes both Isoform 1 and 2 of the alpha chain.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Haptoglobin Capture Antibody 1 x 600µl 10X Human Haptoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Haptoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cardiovascular
- Blood
- Blood Cell Antigens
- RBC Antigens
- Cardiovascular
- Blood
- Serum Proteins
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.Tissue specificity
Expressed by the liver and secreted in plasma.Involvement in disease
AnhaptoglobinemiaSequence similarities
Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.Contains 2 Sushi (CCP/SCR) domains.Cellular localization
Secreted.- Information by UniProt
Alternative names
- Binding peptide
- BP
- Haptoglobin alpha chain
see allDatabase links
- Entrez Gene:3240 Human
- Omim:140100 Human
- SwissProt:P00738 Human
- Unigene:513711 Human
- Unigene:702099 Human
