Overview
Product name
Human Fibronectin ELISA KitSee all Fibronectin kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 3 5% Inter-assay Sample n Mean SD CV% Serum 5 8.3% Sample type
Cell culture supernatant, Milk, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
20.6 pg/mlRange
125 pg/ml -8000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 90%- 117% Milk 102 91%- 111% Serum 93 87%- 102% Cell culture extracts 89 80%- 105% Tissue Extracts 90 80%- 131% EDTA Plasma 86 81%- 94% Cit plasma 99 87%- 120% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:Human, Rhesus monkeyDoes not react with:CowProduct overview
Human Fibronectin ELISA Kit (ab219046) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, cit plasma, edta plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibronectin with 20.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITYThis kit recognizes both native and recombinant human Fibronectin protein in serum, plasma (citrate and EDTA), milk, cell culture supernatant, and cell and tissue extract samples only.
CROSS REACTIVITY Recombinant human Anastelin was prepared within the working range of the assay and assayed for cross reactivity. No cross-reactivity was observed.SPECIES REACTIVITYThis kit recognizes human Fibronectin protein.
Other species reactivity was determined by measuring 1/100,000 serum (mouse, rat, cow) or plasma (Rhesus macaque) samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution.
Notes
Fibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis and nerve regeneration. Differential expression of fibronectin is seen in coronary heart disease, glomerulopathy and tumor cell metastasis. The protein contains binding sites for collagen, heparin and fibrin and is a specific ligand for several integrin adhesion receptors. Fibronectin exists as a dimer or multimer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml 10X Human Fibronectin Capture Antibody 1 x 600µl 10X Human Fibronectin Detector Antibody 1 x 600µl Human Fibronectin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cardiovascular
- Angiogenesis
- Adhesion / ECM
- Extracellular Matrix
- Signal Transduction
- Cytoskeleton / ECM
- Extracellular Matrix
- ECM Proteins
- Fibronectin
- Stem Cells
- Lineage Markers
- Ectoderm
- Stem Cells
- Neural Stem Cells
- Extracellular
- Stem Cells
- Mesenchymal Stem Cells
- Osteogenesis
- Cancer
- Invasion/microenvironment
- ECM
- Extracellular matrix
- Other
- Developmental Biology
- Lineage specification
- Ectoderm
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.Involvement in disease
Glomerulopathy with fibronectin deposits 2Sequence similarities
Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 16 fibronectin type-III domains.Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.Post-translationalmodifications
Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin.Cellular localization
Secreted, extracellular space, extracellular matrix.- Information by UniProt
Alternative names
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see allDatabase links
- Entrez Gene:2335 Human
- Omim:135600 Human
- SwissProt:P02751 Human
- Unigene:203717 Human
