Overview
Product name
Human Galectin-3 ELISA KitSee all Galectin 3 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% A431 extract 5 2.4% Inter-assay Sample n Mean SD CV% A431 extract 3 4.9% Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA PlasmaAssay type
Sandwich (quantitative)Sensitivity
10 pg/mlRange
0.16 ng/ml -10 ng/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 85 81%- 89% Serum 104 99%- 108% Hep Plasma 89 85%- 91% EDTA Plasma 88 83%- 91% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human Galectin-3 ELISA kit has been re-developed with recombinant monoclonal antibodies. This version will be discontinuedwhen inventory is depleted. The new version is available as ab269555.
Human Galectin-3 ELISA Kit (ab188394) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 protein in cell culture supernatant, serum, tissue extracts, edta plasma, hep plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-3 with 10 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is in the Galectin family of carbohydrate-binding proteins and is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Platform
Microplate
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Galectin-3 Capture Antibody 1 x 600µl 10X Human Galectin-3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody DiluentCPI - HAMA Blocker (ab193969) 1 x 6ml Calibrator Diluent 1 1 x 20ml Human Galectin-3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Neuroscience
- Cell Adhesion Proteins
- Nuclear Proteins
- Neuroscience
- Cell Adhesion Proteins
- ECM Proteins
- Epigenetics and Nuclear Signaling
- DNA / RNA
- RNA Processing
- Splicing
- Cell Biology
- Cell Cycle
- Cell differentiation
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Neuroscience target ELISA kits
Relevance
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.Contains 1 galectin domain.Cellular localization
Cytoplasmic, Nuclear and SecretedAlternative names
- 35 kDa lectin
- Carbohydrate-binding protein 35
- CBP 35
see allDatabase links
- Entrez Gene:3958 Human
- Omim:153619 Human
- SwissProt:P17931 Human
- Unigene:531081 Human
