Overview
Product name
Human Cystatin C ELISA KitSee all Cystatin C kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% HeLa extract 5 3.45% Inter-assay Sample n Mean SD CV% HeLa extract 3 4.83% Sample type
Cell culture supernatant, Urine, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
15 pg/mlRange
39 pg/ml -2500 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 97 94%- 101% Plasma 97 95%- 98% Cell culture media 94 85%- 105% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human Cystatin C ELISA kit (ab179883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in human plasma, serum urine and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Cystatin C with 15 pg/mL sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
This kit is knockout validated for specifically detectingcystatin Cin human HAP1 cells.
Notes
Cystatin C fuctions as an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. The CST3 gene is located in the cystatin locus and comprises 3 exons (coding regions, as opposed to introns, non-coding regions within a gene), spanning 4.3 kilo-base pairs. It encodes the most abundant extracellular inhibitor of cysteine proteases. It is found in high concentrations in biological fluids and is expressed in virtually all organs of the body (CST3 is a housekeeping gene). Cystatin C has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. If kidney function and glomerular filtration rate decline, the blood levels of cystatin C rise. Serum levels of cystatin C are a more precise test of kidney function (as represented by the glomerular filtration rate, GFR) than serum creatinine levels.
Platform
Microplate
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human Cystatin C Capture Antibody 1 x 600µl 10X Human Cystatin C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Cystatin C Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cardiovascular
- Blood
- Serum Proteins
- Cell Biology
- Proteolysis / Ubiquitin
- Protease inhibitors
- Other protease inhibitors
- Cardiovascular
- Atherosclerosis
- Thrombosis
- Other
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Cardiovascular ELISA kits
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.Involvement in disease
Defects in CST3 are the cause of amyloidosis type 6 (AMYL6) [MIM:105150]; also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low.Genetic variations in CST3 are associated with age-related macular degeneration type 11 (ARMD11) [MIM:611953]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.Sequence similarities
Belongs to the cystatin family.Post-translationalmodifications
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.Cellular localization
Secreted.- Information by UniProt
Alternative names
- ARMD11
- bA218C14.4 (cystatin C)
- Cst3
see allDatabase links
- Entrez Gene:1471 Human
- Omim:604312 Human
- SwissProt:P01034 Human
- Unigene:304682 Human
