Overview
Product name
Organelle Detection Western Blot CocktailSample type
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Nuclear ExtractsAssay type
QuantitativeSpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
ab133989 contains 4 mAbs each targeting a specific organelle marker. The presence of plasma membrane is determined by Anti-Sodium Potassium ATPase antibody; mitochondrion by Anti-ATP5A antibody; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.
This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.
Tested applications
Suitable for:WBmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 200 µl Organelle Detection Western Blot Cocktail 1 x 200µl Research areas
- Neuroscience
- Neurology process
- Neurodegenerative disease
- Huntington"s disease
- Neuroscience
- Neurology process
- Neurodegenerative disease
- Alzheimer"s disease
- Other
- Tags & Cell Markers
- Subcellular Markers
- Plasma Membrane
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Mitochondria
- Signal Transduction
- Metabolism
- Plasma Membrane
- ATPases
- Signal Transduction
- Metabolism
- Energy Metabolism
- Signal Transduction
- Metabolism
- Mitochondrial
- Neuroscience
- Cell Type Marker
- Neuron marker
- Synapse marker
- Neuroscience
- Neurology process
- Neurogenesis
- Kits/ Lysates/ Other
- Kits
- Cell Metabolism Kits
- Other Metabolism Assay
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of carbohydrates
- Cancer
- Cancer Metabolism
- Response to hypoxia
- Kits/ Lysates/ Other
- Kits
- Cell Metabolism Kits
- Dehydrogenase Kits
- Kits/ Lysates/ Other
- Kits
- Cell Metabolism Kits
- Ion and metal assay kits
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Carbohydrate metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- Metabolism
- Pathways and Processes
- Metabolism processes
- Hypoxia
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Oxidative phosphorylation
- Complex V
- Metabolism
- Types of disease
- Neurodegenerative disease
- Kits/ Lysates/ Other
- Kits
- Antibody Cocktails
- Organelle
Cellular localization
Sodium Potassium ATPase: Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.ATP5A: Mitochondrion inner membrane. Peripheral membrane protein.GAPDH: Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.Histone H3: Nucleus. Chromosome.Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate
- adenosinetriphosphatase
- aging associated gene 9 protein
see allDatabase links
- Entrez Gene:2597 Human
- Entrez Gene:476 Human
- Entrez Gene:480 Human
- Entrez Gene:481 Human
- Entrez Gene:498 Human
- Entrez Gene:8350 Human
- Entrez Gene:8351 Human
- Entrez Gene:8352 Human
see all
