Overview
Product name
Human IL-1 beta ELISA Kit, FluorescentSee all IL-1 beta kitsDetection method
FluorescentPrecision
Intra-assay Sample n Mean SD CV% Media 8 4.8% Inter-assay Sample n Mean SD CV% Media 3 5.6% Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
2.6 pg/mlRange
3.5 pg/ml -3600 pg/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 96%- 100% Serum 103 101%- 105% Hep Plasma 100 99%- 101% EDTA Plasma 93 90%- 96% Cit plasma 86 84%- 88% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanDoes not react with:CowProduct overview
IL-1 beta in vitro CatchPoint®ELISAkit is designed for the quantitative measurement of IL-1 beta protein in human serum, plasma and cell culture supernatants.
This CatchPoint ELISA kit has beenoptimized for Molecular Devices Microplate Readers. Clickherefor a list of recommended Microplate Readers.If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint ELISA Kits is available with all the protocol and analysis settings atwww.softmaxpro.org.
The CatchPoint®ELISAemploys an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPointHRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Notes
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human IL-1beta Capture Antibody 1 x 600µl 10X Human IL-1beta Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 4BI 1 x 6ml Human IL-1beta Lyophilized Recombinant Protein (ab9617) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml Research areas
- Immunology
- Innate Immunity
- Cytokines
- Interleukins
- Cardiovascular
- Atherosclerosis
- Vascular Inflammation
- Inflammatory mediators
- Metabolism
- Types of disease
- Obesity
Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.Tissue specificity
Expressed in activated monocytes/macrophages (at protein level).Sequence similarities
Belongs to the IL-1 family.Post-translationalmodifications
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.Cellular localization
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.- Information by UniProt
Alternative names
- Catabolin
- H1
- IFN beta inducing factor
see allDatabase links
- Entrez Gene:3553 Human
- Omim:147720 Human
- SwissProt:P01584 Human
- Unigene:126256 Human
