Overview
Product name
FirePlex miRNA Panel- Kidney ToxicityDetection method
Flow cytometry-fluorescentAssay type
MultiplexAssay time
4h00mSpecies reactivity
Reacts with:Mouse, Rat, HumanProduct overview
Multiplex miRNA Assay - Kidney Toxicity Panel (ab219508) are the FirePlex® particles designedto profile up to 65 literature curated miRNA for kidney injury and toxicity,miRNAs that have been demonstrated in multiple peer-reviewed studies to be differentially regulated as a part of kidney toxicity or acute kidney injury.
NOTE: FirePlex miRNA panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835)to select the most appropriate Run Buffer for your flow cytometer.
Our multiplex miRNA assays use our FirePlex® particle technology to profile up to 65 miRNAs per well. With the same sensitivity as PCR, they can be used either directlywith 10 µL of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA.
Find a full list of validated sample types, and learn more about the performance and requirements of these assays,here.
These miRNAs have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in plasma, serum, urine, isolated exosomes or total RNA from kidney or kidney-derived cell lines.
Included in the panel are general markers for multiple kidney-related diseases, markers specific to specific kidney states or treatment responses, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls are included to validate assay performance.
It is important to use the normalization procedure in the FirePlex® Analysis Workbench Softwareto identify the miRNAs that can optimally be used for normalization with your samples. Three miRNAs that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel.
Notes
To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab218365 for use with purified RNA or ab218342 for use withbiofluids.
FirePlex® is a registered trade mark in the United States and is an unregistered trade mark elsewhere.
** Hsa = Homo sapiens (human), Mmu = Mus musculus (mouse), Rno = Rattus norvegicus (rat), Ath = Arabidopsis thaliana (arabidposis), Oan = Ornithorhynchus anatinus (platypus), Cel = Caenorhabditis elegans (worm).
#
miRNA 21 name
miRNA Sequence (miRBase V21)
Sequence homology **
MIMAT ID
1
hsa-let-7c-5p
UGAGGUAGUAGGUUGUAUGGUU
Hu, Ms, Rt
MIMAT0000064
2
hsa-let-7e-5p
UGAGGUAGGAGGUUGUAUAGUU
Hu, Ms, Rt
MIMAT0000066
3
hsa-let-7g-5p
UGAGGUAGUAGUUUGUACAGUU
Hu, Ms, Rt
MIMAT0000414
4
hsa-miR-100-5p
AACCCGUAGAUCCGAACUUGUG
Hu, Ms, Rt
MIMAT0000098
5
hsa-miR-10a-5p
UACCCUGUAGAUCCGAAUUUGUG
Hu, Ms, Rt
MIMAT0000253
6
hsa-miR-122-5p
UGGAGUGUGACAAUGGUGUUUG
Hu, Ms, Rt
MIMAT0000421
7
hsa-miR-125b-5p
UCCCUGAGACCCUAACUUGUGA
Hu, Ms, Rt
MIMAT0000423
8
hsa-miR-126-3p
UCGUACCGUGAGUAAUAAUGCG
Hu, Ms, Rt
MIMAT0000445
9
hsa-miR-130a-3p
CAGUGCAAUGUUAAAAGGGCAU
Hu, Ms, Rt
MIMAT0000425
10
hsa-miR-130b-3p
CAGUGCAAUGAUGAAAGGGCAU
Hu, Ms, Rt
MIMAT0000691
11
hsa-miR-132-3p
UAACAGUCUACAGCCAUGGUCG
Hu, Ms, Rt
MIMAT0000426
12
hsa-miR-134-5p
UGUGACUGGUUGACCAGAGGGG
Hu, Ms, Rt
MIMAT0000447
13
hsa-miR-138-5p
AGCUGGUGUUGUGAAUCAGGCCG
Hu, Ms, Rt
MIMAT0000430
14
hsa-miR-140-3p
UACCACAGGGUAGAACCACGG
Hu, Ms, Rt
MIMAT0004597
15
hsa-miR-142-3p
UGUAGUGUUUCCUACUUUAUGGA
Hu, Ms, Rt
MIMAT0000434
16
hsa-miR-143-3p
UGAGAUGAAGCACUGUAGCUC
Hu,Ms
MIMAT0000435
17
hsa-miR-145-5p
GUCCAGUUUUCCCAGGAAUCCCU
Hu, Ms, Rt
MIMAT0000437
18
hsa-miR-146a-5p
UGAGAACUGAAUUCCAUGGGUU
Hu, Ms, Rt
MIMAT0000449
19
hsa-miR-146b-5p
UGAGAACUGAAUUCCAUAGGCU
Hu,Ms
MIMAT0002809
20
mmu-miR-155-5p
UUAAUGCUAAUUGUGAUAGGGGU
Ms, Rt
MIMAT0000165
21
hsa-miR-15a-5p
UAGCAGCACAUAAUGGUUUGUG
Hu,Ms
MIMAT0000068
22
hsa-miR-15b-5p
UAGCAGCACAUCAUGGUUUACA
Hu, Ms, Rt
MIMAT0000417
23
hsa-miR-16-5p
UAGCAGCACGUAAAUAUUGGCG
Hu, Ms, Rt
MIMAT0000069
24
hsa-miR-17-5p
CAAAGUGCUUACAGUGCAGGUAG
Hu, Ms, Rt
MIMAT0000070
25
hsa-miR-181a-5p
AACAUUCAACGCUGUCGGUGAGU
Hu, Ms, Rt
MIMAT0000256
26
mmu-miR-182-5p
UUUGGCAAUGGUAGAACUCACACCG
Ms, Rt
MIMAT0000211
27
rno-miR-183-5p
UAUGGCACUGGUAGAAUUCACU
Hu, Ms, Rt
MIMAT0000860
28
hsa-miR-186-5p
CAAAGAAUUCUCCUUUUGGGCU
Hu, Ms, Rt
MIMAT0000456
29
hsa-miR-18a-5p
UAAGGUGCAUCUAGUGCAGAUAG
Hu, Ms, Rt
MIMAT0000072
30
hsa-miR-191-5p
CAACGGAAUCCCAAAAGCAGCUG
Hu, Ms, Rt
MIMAT0000440
31
hsa-miR-192-5p
CUGACCUAUGAAUUGACAGCC
Hu, Ms, Rt
MIMAT0000222
32
hsa-miR-194-5p
UGUAACAGCAACUCCAUGUGGA
Hu, Ms, Rt
MIMAT0000460
33
hsa-miR-19a-3p
UGUGCAAAUCUAUGCAAAACUGA
Hu, Ms, Rt
MIMAT0000073
34
hsa-miR-200a-3p
UAACACUGUCUGGUAACGAUGU
Hu, Ms, Rt
MIMAT0000682
35
hsa-miR-200c-3p
UAAUACUGCCGGGUAAUGAUGGA
Hu,Ms
MIMAT0000617
36
hsa-miR-203a-3p
GUGAAAUGUUUAGGACCACUAG
Hu, Ms, Rt
MIMAT0000264
37
hsa-miR-206
UGGAAUGUAAGGAAGUGUGUGG
Hu, Ms, Rt
MIMAT0000462
38
hsa-miR-20b-5p
CAAAGUGCUCAUAGUGCAGGUAG
Hu, Ms, Rt
MIMAT0001413
39
hsa-miR-210-3p
CUGUGCGUGUGACAGCGGCUGA
Hu, Ms, Rt
MIMAT0000267
40
mmu-miR-212-3p
UAACAGUCUCCAGUCACGGCCA
Ms, Rt
MIMAT0000659
41
hsa-miR-214-3p
ACAGCAGGCACAGACAGGCAGU
Hu,Ms
MIMAT0000271
42
hsa-miR-21-5p
UAGCUUAUCAGACUGAUGUUGA
Hu, Ms, Rt
MIMAT0000076
43
hsa-miR-218-5p
UUGUGCUUGAUCUAACCAUGU
Hu, Ms, Rt
MIMAT0000275
44
hsa-miR-223-3p
UGUCAGUUUGUCAAAUACCCCA
Hu,Ms
MIMAT0000280
45
hsa-miR-23b-3p
AUCACAUUGCCAGGGAUUACC
Hu, Ms, Rt
MIMAT0000418
46
hsa-miR-26b-5p
UUCAAGUAAUUCAGGAUAGGU
Hu, Ms, Rt
MIMAT0000083
47
hsa-miR-27a-3p
UUCACAGUGGCUAAGUUCCGC
Hu, Ms, Rt
MIMAT0000084
48
hsa-miR-29a-3p
UAGCACCAUCUGAAAUCGGUUA
Hu, Ms, Rt
MIMAT0000086
49
hsa-miR-30a-5p
UGUAAACAUCCUCGACUGGAAG
Hu, Ms, Rt
MIMAT0000087
50
hsa-miR-30c-5p
UGUAAACAUCCUACACUCUCAGC
Hu, Ms, Rt
MIMAT0000244
51
hsa-miR-30e-3p
CUUUCAGUCGGAUGUUUACAGC
Hu, Ms, Rt
MIMAT0000693
52
hsa-miR-320a
AAAAGCUGGGUUGAGAGGGCGA
Hu, Ms, Rt
MIMAT0000510
53
hsa-miR-342-3p
UCUCACACAGAAAUCGCACCCGU
Hu, Ms, Rt
MIMAT0000753
54
hsa-miR-34a-5p
UGGCAGUGUCUUAGCUGGUUGU
Hu, Ms, Rt
MIMAT0000255
55
hsa-miR-34c-5p
AGGCAGUGUAGUUAGCUGAUUGC
Hu, Ms, Rt
MIMAT0000686
56
mmu-miR-378a-3p
ACUGGACUUGGAGUCAGAAGG
Ms, Rt
MIMAT0003151
57
hsa-miR-409-3p
GAAUGUUGCUCGGUGAACCCCU
Hu,Ms
MIMAT0001639
58
hsa-miR-455-5p
UAUGUGCCUUUGGACUACAUCG
Hu, Ms, Rt
MIMAT0003150
59
hsa-miR-484
UCAGGCUCAGUCCCCUCCCGAU
Hu, Ms, Rt
MIMAT0002174
60
hsa-miR-486-5p
UCCUGUACUGAGCUGCCCCGAG
Hu, Ms, Rt
MIMAT0002177
61
mmu-miR-489-3p
AAUGACACCACAUAUAUGGCAGC
Ms, Rt
MIMAT0003112
62
hsa-miR-494-3p
UGAAACAUACACGGGAAACCUC
Hu,Ms
MIMAT0002816
63
hsa-miR-532-3p
CCUCCCACACCCAAGGCUUGCA
Hu, Ms, Rt
MIMAT0004780
64
hsa-miR-93-5p
CAAAGUGCUGUUCGUGCAGGUAG
Hu, Ms, Rt
MIMAT0000093
65
hsa-miR-98-5p
UGAGGUAGUAAGUUGUAUUGUU
Hu, Ms, Rt
MIMAT0000096
66
ath-mir167d
UGAAGCUGCCAGCAUGAUCUGG
Ath
MIMAT0000905
67
cel-mir-70-3p
UAAUACGUCGUUGGUGUUUCCAU
Cel
MIMAT0000042
68
oan-miR-7417-5p
UUCCCCACUCUGAGCACACAGC
Oan
MIMAT0028982
69
x-control
x-control
N/A
70
blank
blank
N/A
Platform
Multiplex
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 48 tests 1 x 96 tests FirePlex®Particle Mix (Circulating) 1 x 1750µl 1 x 3500µl
