Overview
Product name
Y-27632 dihydrochloride, Rho kinase inhibitorDescription
Selective Rho kinase inhibitorBiological description
Y-27632 is a research tool with a wide variety of biological effects, mainly used in the field of stem cell research for maintenance and differentiation.Commonly used as 3D growth matrix component; also a component of brain organoid differentiation media.
How others have used Y-27632 in their research:
Maintenance of tissue culture of iPSCs; 10 μM Y-27632 for 24 hr; Montefiori et al (2018)Reprogramming of breast cancer cells to induced progenitor-like cells (iPLs) ; 2 μM Y27632 ; Yuan et al (2018)Differentiation - Y-27632 enables human embryonic stem cells to survive and differentiate into cortical and basal telencephalic progenitors; Watanabe et al (2007)
*Also available in simple stock solutions (ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
Purity
>99%CAS Number
129830-38-2Chemical structure
Properties
Chemical name
(R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochlorideMolecular weight
320.26Molecular formula
C14H21N3O.2HClPubChem identifier
9901617Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.Solubility overview
Soluble in water to 100 mM and in DMSO to 100mMHandling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
SMILES
Cl.Cl.O=C(Nc1ccncc1)[C@@H]2CC[C@H](CC2)[C@@H](C)NSource
Synthetic
Research areas
- Cell Biology
- Apoptosis
- Intracellular
- Kinases
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Microfilaments
- Actin etc
- Actin Assembly
- Signal Transduction
- Protein Phosphorylation
- Ser / Thr Kinases
- Other Kinases
- Signal Transduction
- Signaling Pathway
- G Protein Signaling
- Small G Proteins
- Ras Family
- Cell Biology
- Cell Cycle
- Cell Division
- Centromere
- Cancer
- Cell cycle
- Cell division
- Cancer
- Signal transduction
- Protein phosphorylation
- Serine/threonine kinases
- Other
- Biochemicals
- Product Range
- Just Add Water
- Biochemicals
- Chemical Type
- Biochemicals
- Biochemicals
- Pharmacology
- Enzymes
- Kinase
- Rho kinase
- Inhibitors
- Biochemicals
- Pharmacology
- Signaling
- Extracellular matrix & cell adhesion
- Rho kinase
- Biochemicals
- Pharmacology
- Signaling
- Stem Cells
- Other
- Biochemicals
- Research Area
- Cancer
- Extracellular matrix & cell adhesion
- Rho kinase
