Overview
Product name
Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymerSee all HRP/DAB detection kit kitsDetection method
ColorimetricAssay type
Micro-PolymerProduct overview
This IHC kit is used to detect rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit ab80437, except that it uses the HRP micro-polymer antibody used during the validation of Abcam"s antibodies inIHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate.
Notes
The previous version of this kit, the EXPOSE Rabbit Specific HRP/DAB Detection IHC Kit, wasused by researchers to stain over 75,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Mouse / anti-Rabbit HRP-DABMicro-polymer IHC Kit ab236466, andHRP-AEC micro-polymer IHC kits.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in theIHC kits and reagents guide.
Tested applications
Suitable for:IHC-Pmore details
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 15 ml 60 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml DAB substrate 1 x 15ml 1 x 60ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml Protein block 1 x 15ml 1 x 60ml Research areas
- Kits/ Lysates/ Other
- Kits
- IHC Kits
Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
