Overview
Product name
Human ALT ELISA KitSee all Alanine Transaminase kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Serum 8 4.5% Sample type
Serum, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
47.34 pg/mlRange
109.38 pg/ml -7000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 117 %- % EDTA Plasma 111 %- % Cit plasma 92 %- % Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human ALT ELISA Kit (ab234578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALT with 47.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only.
Tissue extract samples have not been tested with this kit.
This kit is incompatible with plasma- heparin and cell extract samples.
SPECIES REACTIVITYThis kit recognizes human ALT protein. Other species reactivity not determined. Please contact our Technical Support team for more information.
Notes
ALT (Alanine aminotransferase 1, ALT1) is an aminotransferase that plays a key role in metabolism. It forms pyruvate through the reversible transamination of alanine and 2-oxoglutarate; this reaction is pivotal for gluconeogenesis and amino acid degradation. It is part of a key blood panel diagnostic that is indicative of liver health.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human ALT Capture Antibody 1 x 600µl 10X Human ALT Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human ALT Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS(ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Signal Transduction
- Metabolism
- Amino Acids
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Amino acid metabolism
Function
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.Tissue specificity
Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.Pathway
Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.Cellular localization
Cytoplasm.- Information by UniProt
Alternative names
- AAT1
- Alanine aminotransferase
- Alanine aminotransferase 1
see allDatabase links
- Entrez Gene:2875 Human
- Omim:138200 Human
- SwissProt:P24298 Human
- Unigene:103502 Human
