Overview
Product name
Human GLP1 (7-36) ELISA KitSee all GLP1 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Human serum 8 7% Inter-assay Sample n Mean SD CV% Human serum 3 9% Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
25 pg/mlRange
59.6 pg/ml -1000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Serum 109 105%- 112% Cell culture media 99 93%- 102% Hep Plasma 110 104%- 112% Cit plasma 115 107%- 123% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanPredicted to work with:Sheep, Horse
Product overview
Human GLP1 (7-36) ELISA kit (ab184857) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GLP-1(7-36) active peptide protein in human serum, plasma and culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GLP1 with25 pg/mL sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:-Single-wash protocol reduces assay time to 90 minutes or less-High sensitivity, specificity and reproducibility from superior antibodies-Fully validated in biological samples-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Notes
GLP-1 (Glucagon like peptide 1) is part of the group of incretin hormones that are secreted by the gastrointestinal tract in response to food intake to assist glucose stimulated insulin secretion and glucagon suppression. GLP-1 is a 30 aminoacid peptide cleaved from proglucagon and released by the L-cells of the distal ileum. The intracellular precursor of GLP-1 (1-37) is cleaved to form the active peptides GLP-1 (7-37) and GLP-1 (7-36)NH2. The active peptides bind to the GLP-1 receptor (GLP-1r) expressed in the pancreatic beta cell and are quickly metabolized by the enzyme dipeptidyl peptidase IV (DPP-IV) to form the peptide GLP-1 (9-36), which has no insulin stimulating activity. Binding of active GLP-1 to the receptor, increases cAMP levels and potentiates insulin secretion via Protein Kinase A (PKA) and the cAMP-regulated nucleotide exchange factor (Epac2).GLP-1 and its receptor are also suggested to play a role in the central nervous systems as mediators of satiety. Intracerebroventricular GLP-1 has been shown to induce c-FOS activity in the hypothalamus and the central nucleus of the amygdala, both of which are important in the regulation of appetite.
Platform
Microplate
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Human GLP1 (7-36) Capture Antibody 1 x 600µl 10X Human GLP1 (7-36) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody DiluentCPI - HAMA Blocker (ab193969) 1 x 6ml Human GLP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Carbohydrate metabolism
Relevance
Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.Cellular localization
Secreted.Alternative names
- GCG
- Glicentin
- Glicentin-related polypeptide
see allDatabase links
- Entrez Gene:2641 Human
- Omim:138030 Human
- SwissProt:P01275 Human
